Recombinant Danio rerio Pituitary homeobox 2 (pitx2)

Code CSB-YP894781DIL
MSDS
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Source Yeast
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Code CSB-EP894781DIL
MSDS
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Source E.coli
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Code CSB-EP894781DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894781DIL
MSDS
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Source Baculovirus
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Code CSB-MP894781DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
pitx2; pitx2a; pitx2b; zgc:110508; Pituitary homeobox 2; Homeobox protein PITX2; Paired-like homeodomain transcription factor 2
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-314
Target Protein Sequence
MTSMKDPLSL DHHHHNHHVT GSKHAPLSMA SSLQPLQRSV DSKHRLDVHT VSDTSSPESV EKEKGQSKNE DSNDDPSKKK RQRRQRTHFT SQQLQELEAT FQRNRYPDMS TREEIAVWTN LTEARVRVWF KNRRAKWRKR ERNQQAELCK NGFGPQFNGL MQPYDDMYPS YTYNNWAAKG LTSASLSTKS FPFFNSMNVN PLSSQTMFSP PNSISSMSMS SSMVPSAVTG VPGSSLNSLN NLNNLSNPSL NSGVPTPACP YAPPTPPYVY RDTCNSSLAS LRLKAKQHSS FGYASVQNPA SNLSACQYAV DRPV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Isoform a and isoform c differentially regulate mesendoderm formation and asymmetric organ development. Both isoforms regulate left-right development, but isoform a has a stronger effect on heart laterality.
Gene References into Functions
  1. Late zygotic oep mutants have strongly reduced or absent pitx2 expression in the lateral plate mesoderm (LPM), but this expression can be rescued to strong levels by restoring oep in midline structures only. PMID: 27821532
  2. asymmetric expression of fatty acid elongase 6 gene is controlled by left right Nodal signaling and is independent of Pitx2 function in zebrafish PMID: 27297886
  3. This study found PITX2 to be decreased in patients with sustained Atrial Fibrillation, suggesting a PITX2 loss of function mechanism in Atrial Fibrillation. PMID: 26021244
  4. Antagonism between Nodal and Pitx2c activities sets an upper limit on parapineal cell numbers. Restricting parapineal cell number is crucial for the correct elaboration of epithalamic asymmetry. PMID: 24598158
  5. Pitx2, implicated in left-right asymmetry, possessed appropriate 'atypical' Pegasus binding sites in its promoter. PMID: 23499657
  6. Data indicate that retinoic acid (RA) induces the expression patterns of genes normally expressed in the posterior tooth-forming region, such as pitx2 and dlx2b. PMID: 22942074
  7. Pitx2 is essential for proper eye and craniofacial development in zebrafish. PMID: 22303467
  8. Retinoic acid regulation of pitx2 is essential for coordinating interactions among neural crest, mesoderm, and developing eye. PMID: 22125274
  9. Zebrafish pitx2 demonstrates conserved expression during ocular and craniofacial development. Thirteen conserved noncoding sequences positioned within a gene desert as far as 1.1 Mb upstream of the human PITX2 PMID: 20881290

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
Isoform a and isoform c have distinct expression patterns during early mesendoderm development; isoform c is expressed symmetrically in presumptive mesendoderm during late blastula stages, and in both the anterior (polster) and posterior prechordal plate
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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