Recombinant Danio rerio Proliferating cell nuclear antigen(pcna)

Code CSB-YP017621DIL
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Source Yeast
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Code CSB-EP017621DIL
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Source E.coli
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Code CSB-EP017621DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP017621DIL
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Source Baculovirus
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Code CSB-MP017621DIL
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names pcna
Uniprot No. Q9PTP1
Alternative Names pcna; Proliferating cell nuclear antigen; PCNA
Species Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region 1-260
Target Protein Sequence MFEARLVQGS ILKKVLEALK DLITEACWDV SSSGISLQSM DSSHVSLVQL TLRSDGFDSY RCDRNLAMGV NLSSMSKILK CAGNEDIITL RAEDNADALA LVFETLNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSQIGDAVMI SCAKDGVKFS ASGELGTGNI KLSQTSNVDK EDEAVTIEMN EPVQLIFALN YLNFFTKATP LSKTVTLSMS ADIPLVVEYK IADMGHVKYY LAPKIDEESS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.
Gene References into Functions
  1. The expression of telomerase activity and TERT in retina implies that telomerase has functions other than the elongation of telomere. These findings could provide new insights on telomerase function in the nervous system. PMID: 18157659
  2. intestinal clock controls the expression of key cell cycle regulators, such as cdc2, wee1, p21, PCNA and cdk2, but only weakly influences cyclin B1, cyclin B2 and cyclin E1 expression. PMID: 24013905
Subcellular Location Nucleus
Protein Families PCNA family
Database Links

KEGG: dre:30678

STRING: 7955.ENSDARP00000070780

UniGene: Dr.35605

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