Recombinant Danio rerio Protein Wnt-5b (wnt5b)

Code CSB-YP852827DIL
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Source Yeast
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Code CSB-EP852827DIL
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Source E.coli
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Code CSB-EP852827DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP852827DIL
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Source Baculovirus
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Code CSB-MP852827DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
wnt5b
Uniprot No.
Alternative Names
wnt5b; wnt-5; wnt5; wnt5a; si:ch73-211c7.1; Protein Wnt-5b
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
22-363
Target Protein Sequence
QLLVVANSW WSLAMNPIQR PEMYIIGAQP LCSQLTGLSQ GQRKLCQLYQ DHMVYIGEGA KTGIKECQYQ FRQRRWNCST VDNTSVFGRV MHIGSRETAF TYAVSAAGVV NAVSRACREG ELSTCGCSRA ARPRDLPRDW LWGGCGDNVN YGYRFAREFV DAREREKNYP RGSVEHARTL MNLQNNEAGR MAVYNLANVA CKCHGVSGSC SLKTCWLQLA DFRRVGEFLK EKYDSAAAMR INRRGKLELV NNRFNPPTGE DLVYIDPSPD YCLRNETTGS LGTQGRLCNK TSEGMDGCEL MCCGRGYDQF KTYKHERCHC KFHWCCYVKC KRCTSLVDQF VCK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. Required during embryogenesis for extension of the primary anterior-posterior axis. Regulates convergent extension movements and hypaxial myogenesis during gastrulation via activation of non-canonical Wnt signaling.
Gene References into Functions
  1. the chaperon Wls and its ligands Wnt9a and Wnt5b are expressed in the ectoderm, whereas juxtaposed chondrocytes express Frzb and Gpc4. PMID: 27287801
  2. disruption of wls resulted in a significant loss of craniofacial bone, whereas lack of gpc4, wnt5b and wnt9a resulted in severely delayed endochondral ossification. PMID: 27908786
  3. novel mechanism for Wnt5b and Gpc4 regulation of chondrocyte behavior that is independent of the core Wnt/PCP molecules and differs from their collaborative action of controlling cell movements during gastrulation PMID: 26459057
  4. In embryos overexpressing lbx1b, wnt5b, a ligand of the non-canonical Wnt/planar cell polarity (PCP) pathway, was significantly downregulated. PMID: 26820155
  5. Swap70b and Def6a delineate Wnt11 and Wnt5b signalling pathways and have roles in convergent and extension cell movement during zebrafish gastrulation PMID: 24177263
  6. CNBP up-regulates tbx2b and smarca5, and down-regulates wnt5b gene expression. PMID: 23667590
  7. def6 morphants phenocopy Wnt5b mutants and ectopic overexpression of def6 essentially rescues Wnt5b morphants, indicating a novel role for def6 as a central GEF downstream of Wnt5b signaling PMID: 22039507
  8. identify rgs3 as having an overlapping expression pattern with wnt5b in zebrafish and reveal that individual knockdown of either rgs3 or wnt5b gene function produces similar somite patterning defects. PMID: 20628572
  9. Data suggest that full-length Ryk conveys Wnt5b signals in a directional manner during gastrulation. PMID: 20660632
  10. Ppt/Wnt5 has a role in regulating cell shape and movement during zebrafish gastrulation PMID: 12676324
  11. Data suggest that the Wnt-5 loss-of-function defect is consistent with calcium modulation having an antagonistic interaction with Wnt/beta-catenin signaling. PMID: 12952939
  12. conserved role of a Wnt5/Fz2 signaling pathway in islet formation during pancreatic development. PMID: 16246260

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Database Links

KEGG: dre:30105

STRING: 7955.ENSDARP00000106303

UniGene: Dr.389

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