Recombinant Danio rerio Ubiquitin-like-conjugating enzyme ATG3(atg3)

Code CSB-YP761343DIL
Size Pls inquire other sizes
Source Yeast
Code CSB-EP761343DIL
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Source E.coli
Code CSB-EP761343DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP761343DIL
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Source Baculovirus
Code CSB-MP761343DIL
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names atg3
Uniprot No. Q6PFS7
Species Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region 1-317
Target Protein Sequence MQNVINSVKG TALGVAEFLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWKWASGEE AKVKPYLPND KQFLLTRNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT FHNSGVTGVT EAVREISLDN KDNMNMNVKT GACGNSGDDD DDEEGEAADM EEYEESGLLE TDDATLDTSK MADLSKTKAE AGGEDAILQT RTYDLYITYD KYYQTPRLWL FGYDEDRQPL TVDQMYEDIS QDHVKKTVTI ENHPNLPPPA MCSVHPCRHA EVMKKIIETV AEGGGELGVH MYLLIFLKFV QAVIPTIEYD YTRHFTM
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of atg8-like proteins (gabarap, gabarapl1, gabarapl2 or map1lc3a). The atg12-atg5 conjugate plays a role of an E3 and promotes the transfer of atg8-like proteins from atg3 to phosphatidylethanolamine (PE). This step is required for the membrane association of atg8-like proteins. The formation of the atg8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of atg12 to itself, atg12 conjugation to atg3 playing a role in mitochondrial homeostasis but not in autophagy. atg7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with atg3 (By similarity).
Subcellular Location Cytoplasm
Protein Families ATG3 family
Database Links

KEGG: dre:336632

STRING: 7955.ENSDARP00000041303

UniGene: Dr.1496

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