Recombinant Dog Pro-epidermal growth factor (EGF), partial

Code CSB-YP859788DO
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Source Yeast
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Code CSB-EP859788DO
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Source E.coli
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Code CSB-EP859788DO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859788DO
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Source Baculovirus
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Code CSB-MP859788DO
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
EGF
Uniprot No.
Alternative Names
EGFPro-epidermal growth factor; EGF) [Cleaved into: Epidermal growth factor]
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture. Magnesiotropic hormone that stimulates magnesium reabsorption in the renal distal convoluted tubule via engagement of EGFR and activation of the magnesium channel TRPM6.
Gene References into Functions
  1. FGF2 and EGF Are Required for Self-Renewal and Organoid Formation of Canine Normal and Tumor Breast Stem Cells PMID: 27632571
  2. epidermal growth factor, transforming growth factor-alpha and EGF receptor may be involved in testicular maturation and function in the dog PMID: 26498203
  3. Matrix stiffening significantly sensitizes cells to EGF and enables the loss of contact inhibition. PMID: 21429934
  4. EGF-based signaling cascades phosphorylate Cav1 and have roles in caveolae assembly PMID: 16332692
  5. In malignant tumours (non-IMC and IMC), a strong correlation was observed between EGF and: progesterone, 17beta-estradiol, androstenedione, and dehydroepiandrosterone PMID: 19429455

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Database Links
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