Recombinant Drosophila melanogaster Caspase-8 (Dredd)

Code CSB-YP811591DLU
MSDS
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Source Yeast
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Code CSB-EP811591DLU
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Source E.coli
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Code CSB-EP811591DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP811591DLU
MSDS
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Source Baculovirus
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Code CSB-MP811591DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Dredd
Uniprot No.
Alternative Names
Dredd; DCP2; CG7486; Caspase-8; EC 3.4.22.61; Death-related ced-3/NEDD2-like protein) [Cleaved into: Caspase-8 subunit p15; Caspase-8 subunit p10]
Species
Drosophila melanogaster (Fruit fly)
Expression Region
243-400
Target Protein Sequence
YCSTQIDA LKLTRENAGI ALIINQQKFH RNVSRDNMKF LSPDPLRRRD GTDVDKERLI EVFSSMGYNV EAYDNVDHMG IIERIRSACD RSLVRDSLVV FILSHGFEEA VYASNSIAMK ITDIEDLLCS YDTLYYKPKL LIIQACQEKL VHKKKPNELF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Effector of the programmed cell death (PCD) activators rpr, grim and hid. May play an apoptotic role in the germline as well as soma. Fadd interacts with Dredd to promote cleavage of Dredd and is necessary and sufficient for enhancing Dredd-induced apoptosis. Plays a role in the innate immune response. Required for resistance to Gram-negative bacterial infection. Diap2-mediated ubiquitination of Dredd is critical for processing of imd and rel and the subsequent expression of antimicrobial genes such as DptA.
Gene References into Functions
  1. interdomain cleavage of Dredd is not required for Imd or Relish processing and is not observed during immune stimulation PMID: 24891502
  2. The authors show that stimulation of the Drosophila pattern recognition protein PGRP-LCx induces DIAP2-dependent polyubiquitylation of the initiator caspase DREDD. PMID: 22549468
  3. Dredd is an essential component of the IMD pathway required for the full activation of IMD/dJNK in cell culture and in vivo PMID: 21730059
  4. interaction with kep1 and effect of kep mutants on balance of dredd isoforms PMID: 12563030
  5. Dnr1 blocks ectopic Dredd-mediated induction of an NF-kappa B reporter. PMID: 17341581

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase C14A family
Tissue Specificity
Constitutively expressed in fat bodies of larvae and adults.
Database Links
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