Recombinant Drosophila melanogaster Fork head domain transcription factor slp1 (slp1)

Code CSB-YP333661DLU
MSDS
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Source Yeast
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Code CSB-EP333661DLU
MSDS
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Source E.coli
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Code CSB-EP333661DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP333661DLU
MSDS
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Source Baculovirus
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Code CSB-MP333661DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
slp1
Uniprot No.
Alternative Names
slp1; FD6; CG16738; Fork head domain transcription factor slp1; Sloppy paired locus protein 1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-322
Target Protein Sequence
MVKSEMEFKS NFSIDAILAK KPINTATQPI KTEPVHHHHQ YVHPYSNSDG ELSASEDFDS PSRTSTPMSS AAESLSSQNN DKLDVEFDDE LEDQLDEDQE SEDGNPSKKQ KMTAGSDTKK PPYSYNALIM MAIQDSPEQR LTLNGIYQYL INRFPYFKAN KRGWQNSIRH NLSLNKCFTK IPRSYDDPGK GNYWILDPSA EEVFIGETTG KLRRKNPGAS RTRLAAYRQA IFSPMMAASP YGAPAPSYGY PAVPFAAAAA AALYQRMNPA AYQAAYQQMQ YQQAPQAHHH QAPHPAQMQG YPPQLNAELF QRMQFFGKFP SS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor involved in segmentation. Required for the formation of the mandibular lobe. Different levels of slp activity seem to be required in different segments.
Gene References into Functions
  1. slp1 and slp2 are coordinately regulated by 8 stripe enhancers surrounding slp1. PMID: 22178246
  2. abdominal Hox proteins work directly with two segmentation proteins, Sloppy paired and Engrailed, to repress the Hox target gene Distalless in anterior and posterior compartments, respectively; segmentation proteins can function as Hox cofactors PMID: 15470419
  3. Results suggest that sloppy-paired 1 functions as a gap gene-like repressor, and contains a protein motif which mediates binding to the transcriptional corepressor Groucho. PMID: 15581884
  4. Taken together, these data show that Slp1/Foxg1 regulate glial cell fates by inhibiting Gcm function PMID: 17091489
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in the posterior half of each parasegment just anterior to the parasegmental boundary.
Database Links

KEGG: dme:Dmel_CG16738

STRING: 7227.FBpp0077188

UniGene: Dm.4659

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