Recombinant Drosophila melanogaster Guanine nucleotide exchange factor MSS4 homolog (CG7787)

Code CSB-YP892865DLU
MSDS
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Source Yeast
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Code CSB-EP892865DLU
MSDS
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Source E.coli
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Code CSB-EP892865DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892865DLU
MSDS
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Source Baculovirus
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Code CSB-MP892865DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CG7787
Uniprot No.
Alternative Names
strat; CG7787; Guanine nucleotide exchange factor MSS4 homolog; Guanine nucleotide exchange factor stratum
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-122
Target Protein Sequence
MTEEADFSEQ ITDGKNKSNV RCQFCNCLML KAQEGTYNQE EVDVPLMTQK QDRTADSLNS EPLKDFWLVK DMMTFENIGF SNTVDGRKFL VCADCERGPV GYHDLSTRHC YLALKRVVHK DT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Guanine-nucleotide-releasing protein that acts on members of the sec4/ypt1/rab subfamily such as Rab8. During egg development, essential for establishing and maintaining epithelial cell polarity by regulating the correct polarized deposition of basal membrane (BM) proteins such as trol/Pcan and vkg/Coll IV to the basal surface of follicular epithelial (FE) cells. Likely to function by restricting the activity of the vesicle transport regulator Rab8 to the basal membrane, and thus directs BM protein-containing vesicles to the basal side of the FE cells. This function is independent of the Crag/Rab10 regulation of polarized BM protein secretion in the FE.
Subcellular Location
Basal cell membrane; Peripheral membrane protein.
Protein Families
DSS4/MSS4 family
Database Links

KEGG: dme:Dmel_CG7787

STRING: 7227.FBpp0079221

UniGene: Dm.6826

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