Recombinant Drosophila melanogaster Integrin alpha-PS3 (scb), partial

Code CSB-YP526130DLU
MSDS
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Source Yeast
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Code CSB-EP526130DLU
MSDS
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Source E.coli
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Code CSB-EP526130DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP526130DLU
MSDS
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Source Baculovirus
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Code CSB-MP526130DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
scb
Uniprot No.
Alternative Names
scb; alphaPS3; Vol; CG8095; Integrin alpha-PS3; Position-specific antigen subunit alpha-3; Protein scab; Protein volado) [Cleaved into: Integrin alpha-PS3 heavy chain; Integrin alpha-PS3 light chain]
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Integrin alpha-PS3/beta-PS is a receptor for laminin. Also binds to wb. Important during embryogenesis for the development of the trachea, dorsal vessel and salivary gland, as well as for dorsal closure. Required for short-term memory processes. Minor involvement in the establishment of the oocyte anterior-posterior length. Plays a role in timely border cell migration during oogenesis, probably mediated by JNK signaling. Integrin alpha-PS3/Itgbn is required for effective phagocytosis of apoptotic cells during embryonic development and for the phagocytic elimination of S.aureus by mediating the binding of S.aureus peptidoglycan to larval hemocytes, which probably activates a signaling pathway involving Rac1 and Rac2. Integrin alpha-PS3/Itgbn also regulates Fak activity during neuromuscular junction (NMJ) growth and is required for its activation in presynapsis of NMJs. Seems to be dispensable for major morphogenetic processes.
Gene References into Functions
  1. We identify a role for the alphaPS3, betaPS1 Integrin dimer in both the early establishment of the apical, pre-luminal domain, as well as a requirement upon Robo for maintained apicalisation of proteins required for lumen formation. PMID: 22353787
Subcellular Location
Apical cell membrane; Single-pass type I membrane protein. Lateral cell membrane; Single-pass type I membrane protein. Cytoplasm. Note=Apical membrane localization in primordial dorsal-appendage cells at oogenesis stages 10B and 11. Later, weakly expressed in lateral membrane of cells surrounding the outgrowing dorsal appendages.
Protein Families
Integrin alpha chain family
Tissue Specificity
Expressed in embryonic and larval hemocytes (at protein level). Expressed in tissues undergoing invagination, tissue movement and morphogenesis such as salivary gland, trachea, midgut endoderm, dorsal vessel, midline of the ventral nerve cord, amnioserosa
Database Links

KEGG: dme:Dmel_CG8095

STRING: 7227.FBpp0086501

UniGene: Dm.636

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