Recombinant Drosophila melanogaster Vesicle-fusing ATPase 2 (Nsf2), partial

Code CSB-YP346160DLU
MSDS
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Source Yeast
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Code CSB-EP346160DLU
MSDS
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Source E.coli
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Code CSB-EP346160DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP346160DLU
MSDS
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Source Baculovirus
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Code CSB-MP346160DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nsf2
Uniprot No.
Alternative Names
Nsf2; CG33101; Vesicle-fusing ATPase 2; EC 3.6.4.6; N-ethylmaleimide-sensitive fusion protein 2; NEM-sensitive fusion protein 2; Vesicular-fusion protein NSF2; dNsf-2; NSF-2
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin.
Gene References into Functions
  1. CCM3 and GCKIII prevent tube dilation in Drosophila trachea; tube dilation is suppressed by reduction of NSF2 PMID: 23763949
  2. genetic analysis of suppressors of NSF2 neuromuscular junction overgrowth PMID: 15834148
  3. NSF2(E/Q) larval synapses indicate the NMJ overgrowth phenotype is present at the embryonic neuromuscular synapse. At the ultrastructural level, considerable alterations in the number and distribution of synapses and active zones were found. PMID: 15912502
  4. These data are consistent with a model in which actin filaments promote vesicle mobility and suggest that N-ethylmaleimide sensitive factor participates in establishing or maintaining this population of actin. PMID: 16914524
  5. Interaction of cytoskeleton genes with NSF2-induced neuromuscular junction overgrowth suggests that NSF may function near the top of the actin regulatory pathway. PMID: 17139674

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Subcellular Location
Cytoplasm.
Protein Families
AAA ATPase family
Tissue Specificity
Nervous system and secretory tissues.
Database Links

KEGG: dme:Dmel_CG33101

STRING: 7227.FBpp0082346

UniGene: Dm.6688

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