Recombinant Dual specificity mitogen-activated protein kinase kinase mek-2 (mek-2)

Code CSB-YP604623CXY
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Source Yeast
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Code CSB-EP604623CXY
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Source E.coli
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Code CSB-EP604623CXY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP604623CXY
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Source Baculovirus
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Code CSB-MP604623CXY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
mek-2
Uniprot No.
Alternative Names
mek-2; Y54E10BL.6; Dual specificity mitogen-activated protein kinase kinase mek-2; MAP kinase kinase mek-2; EC 2.7.12.2
Species
Caenorhabditis elegans
Expression Region
1-387
Target Protein Sequence
MSSGKRRNPL GLSLPPTVNE QSESGEATAE EATATVPLEE QLKKLGLTEP QTQRLSEFLQ VKEGIKELSE DMLQTEGELG HGNGGVVNKC VHRKTGVIMA RKLVHLEIKP SVRQQIVKEL AVLHKCNSPF IVGFYGAFVD NNDISICMEY MDGLSLDIVL KKVGRLPEKF VGRISVAVVR GLTYLKDEIK ILHRDVKPSN MLVNSNGEIK LCDFGVSGML IDSMANSFVG TRSYMAPERL TGSHYTISSD IWSFGLSLVE LLIGRYPVPA PSQAEYATMF NVAENEIELA DSLEEPNYHP PSNPASMAIF EMLDYIVNGP PPTLPKRFFT DEVIGFVSKC LRKLPSERAT LKSLTADVFF TQYADHDDQG EFAVFVKGTI NLPKLNP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions in the let-60 Ras signaling pathway; acts downstream of lin-45 raf kinase, but before the sur-1/mpk-1 gene product in controlling vulval cell differentiation. Required for progression of developing oocytes through the pachytene stage. Plays a role in responses to M.nematophilum-mediated bacterial infection by promoting tail swelling and preventing constipation. Involved in fluid homeostasis. Positively regulates lifespan upstream of mpk-1.
Gene References into Functions
  1. C. elegans ERK kinase MPK-1 or ERK phosphorylating/activating kinase MEK-2 constitutively activates native ClC anion channel CLH-3b upstream from Gck-3. PMID: 21160027
Protein Families
Protein kinase superfamily, STE Ser/Thr protein kinase family, MAP kinase kinase subfamily
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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