Name: Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K)
Brand: CUSABIO
SKU: CSB-EP362675ECC(M)

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

stxA2

Uniprot No.

P09385

Research Area

Neuroscience

Alternative Names

Verocytotoxin 2 subunit A Verotoxin 2 subunit A rRNA N-glycosidase 2

Species

Enterobacteria phage 933W (Bacteriophage 933W)

Source

E.coli

Expression Region

23-319aa(E189K)

Target Protein Sequence

REFTIDFSTQQSYVSSLNSIRTEISTPLEHISQGTTSVSVINHTPPGSYFAVDIRGLDVYQARFDHLRLIIEQNNLYVAGFVNTATNTFYRFSDFTHISVPGVTTVSMTTDSSYTTLQRVAALERSGMQISRHSLVSSYLALMEFSGNTMTRDASRAVLRFVTVTAKALRFRQIQREFRQALSETAPVYTMTPGDVDLTLNWGRISNVLPEYRGEDGVRVGRISFNNISAILGTVAVILNCHHQGARSVRAVNEESQPECQITGDRPVIKINNTLWESNTAAAFLNRKSQFLYTTGK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

40.6 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Customer Reviews and Q&A

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■ Q&A

Is the protein CSB-EP362675ECC(M) endotoxin-free? If not, what would be the cost for endotoxin-removal?

The recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K) has not undergone endotoxin removal treatment, but we can try to provide free endotoxin removal services to make the endotoxin content less than 1.0 EU/ug. Successful endotoxin removal takes 3-7 working days.

What serogroup does this belong to?

CSB-EP362675ECC(M)
https://www.uniprot.org/uniprotkb/P09385/entry
It belongs to Escherichia coli serotype O157:H7.

Target Background

Function

The A subunit is responsible for inhibiting protein synthesis through the catalytic inactivation of 60S ribosomal subunits. After endocytosis, the A subunit is cleaved by furin in two fragments, A1 and A2: A1 is the catalytically active fragment, and A2 is essential for holotoxin assembly with the B subunits.

Subcellular Location

Secreted.

Protein Families

Ribosome-inactivating protein family

Database Links

KEGG: vg:1261950

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Code	CSB-EP362675ECC(M)
Abbreviation	Recombinant Enterobacteria phage 933W stxA2 protein (E189K)
MSDS	MSDS Datasheet
Size	$388
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Enterobacteria phage 933W Shiga-like toxin 2 subunit A (stxA2) (E189K)

Product Details

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Target Background

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