To prepare this Recombinant EBV B95-8 BDLF3 protein, the recombinant DNA was required, which was generated by fusing the BDLF3 gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this BDLF3 protein production. After purification, a premium BDLF3 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+.
BDLF3 is a protein encoding gene that provides an instruction in making a protein named glycoprotein BDLF3. This gene is predicted to code for a glycoprotein on the basis that it contains sequences with signal peptide and transdomain characteristics and nine potential N-linked glycosylation sites. The protein encoded by this gene is a highly glycosylated protein of Epstein-Barr virus (EBV). It does not have a homolog in the alpha- and betaherpesviruses.
||Greater than 85% as determined by SDS-PAGE.
||BDLF3; Glycoprotein BDLF3
||Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
|Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
|| If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
||We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
||Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
||The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
||3-7 business days
||Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
|Datasheet & COA
|| Please contact us to get it.