Recombinant Epstein-Barr virus Glycoprotein BDLF3(BDLF3),partial

In Stock
Code CSB-EP365881EFA1
Size $2466 How to order?
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365881EFA1 could indicate that this peptide derived from E.coli-expressed Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) BDLF3.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP365881EFA1 could indicate that this peptide derived from E.coli-expressed Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4) BDLF3.
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Product Details

Description

To prepare this Recombinant EBV B95-8 BDLF3 protein, the recombinant DNA was required, which was generated by fusing the BDLF3 gene with N-terminal 10xHis tag and C-terminal Myc tag sequence. Once the recombinant DNA was amplified and purified, a protein expression system, E.coli, was needed for this BDLF3 protein production. After purification, a premium BDLF3 recombinant proteinwas obtained. According to SDS-PAGE, its purity turns out to be 85%+.

BDLF3 is a protein encoding gene that provides an instruction in making a protein named glycoprotein BDLF3. This gene is predicted to code for a glycoprotein on the basis that it contains sequences with signal peptide and transdomain characteristics and nine potential N-linked glycosylation sites. The protein encoded by this gene is a highly glycosylated protein of Epstein-Barr virus (EBV). It does not have a homolog in the alpha- and betaherpesviruses.

Purity Greater than 85% as determined by SDS-PAGE.
Target Names BDLF3
Uniprot No. P03224
Research Area Others
Alternative Names BDLF3; Glycoprotein BDLF3
Species Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)
Source E.coli
Expression Region 29-186aa
Target Protein Sequence SSTASAGNVTGTTAVTTPSPSASGPSTNQSTTLTTTSAPITTTAILSTNTTTVTFTGTTVTPVPTTSNASTINVTTKVTAQNITATEAGTGTSTGVTSNVTTRSSSTTSATTRITNATTLAPTLSSKGTSNATKTTAELPTVPDERQPSLSYGLPLWT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 22.7 kDa
Protein Length Partial
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Gene References into Functions
  1. BDLF3 both enhanced internalization of surface major histocompatibility complex molecules and reduced the rate of their appearance at the cell surface. PMID: 26468525
Subcellular Location Membrane; Single-pass membrane protein.
Protein Families Epstein-Barr virus BDLF3 protein family
Database Links

KEGG: vg:3783694

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