Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

BHRF1

Uniprot No.

P03182

Research Area

others

Alternative Names

BHRF1Apoptosis regulator BHRF1; Early antigen protein R; EA-R; Nuclear antigen

Species

Epstein-Barr virus (strain B95-8) (HHV-4) (Human herpesvirus 4)

Source

E.coli

Expression Region

1-142aa

Target Protein Sequence

MAYSTREILLALCIRDSRVHGNGTLHPVLELAARETPLRLSPEDTVVLRYHVLLEEIIERNSETFTETWNRFITHTEHVDLDFNSVFLEIFHRGDPSLGRALAWMAWCMHACRTLCCNQSTPYYVVDLSVRGMLEASEGLDG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

19.8kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The expression region of this recombinant Epstein-Barr virus BHRF1 covers amino acids 1-142. The expected molecular weight for the BHRF1 protein is calculated to be 19.8 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag was smoothly integrated into the coding gene of BHRF1, which enables a simple process of detecting and purifying the BHRF1 recombinant protein in the following steps.

The Epstein-Barr virus (EBV) apoptosis regulator BHRF1 is a homolog of the cellular Bcl-2 protein, which plays a crucial role in regulating apoptosis and inhibiting the intrinsic apoptotic pathway. Similarly, BHRF1 functions as an anti-apoptotic protein in EBV-infected cells. By interacting with pro-apoptotic factors and preventing mitochondrial outer membrane permeabilization, BHRF1 helps the virus evade the host immune response and establish persistent infections. It is particularly involved in blocking the apoptotic response induced by various cellular stress signals, contributing to the survival of infected cells. Understanding the mechanisms employed by BHRF1 in modulating apoptosis is crucial for elucidating the pathogenesis of EBV-associated diseases and may have implications for the development of antiviral strategies.

Target Background

Function

Prevents premature death of the host cell during virus production, which would otherwise reduce the amount of progeny virus. Acts as a host B-cell leukemia/lymphoma 2 (Bcl-2) homolog, and interacts with pro-apoptotic proteins to prevent mitochondria permeabilization, release of cytochrome c and subsequent apoptosis of the host cell.

Subcellular Location

Host membrane; Single-pass membrane protein. Host mitochondrion. Note=also observed in the perinuclear region of the cell.

Protein Families

Bcl-2 family

Database Links

KEGG: vg:3783706

Code	CSB-EP314488EFC
Abbreviation	Recombinant Epstein-Barr virus BHRF1 protein, partial
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Epstein-Barr virus Apoptosis regulator BHRF1 (BHRF1), partial

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