Code | CSB-EP357355ENV |
Size | US$388 |
Order now | |
Image | |
Have Questions? | Leave a Message or Start an on-line Chat |
The Recombinant E.coli K12 mutT protein is a protein encoded by recombinant DNA that was cloned in an expression vector that supported the expression of mutT gene. This recombinant mutT protein was expressed in the host. The expression region is 1-129aa of the E.coli K12 mutT. In the production, the expression vector contains N-terminal 6xHis-SUMO tag. Every production step was performed with a strict QC system. The purity of this protein is 90%+ determined by SDS-PAGE.
The MutT protein in Escherichia coli removes 8-oxo-deoxyguanosine triphosphate (8-oxo-dGTP) and 8-oxo-guanosine triphosphate (8-oxo-GTP) from the nucleotide pools precluding incorporation into DNA and RNA. 8-oxo-dGTP is a potent mutagenic nucleotide that is readily incorporated into DNA opposite template C or A, with a preference for template A. Therefore, by cleaning the 8-oxo-dGTP pool, MutT reduces spontaneous transversion mutations ∼1000-fold. In addition, MutT can also act on 8-oxo-guanosine triphosphate (8-oxo-GTP) and convert it to 8-oxo-GMP averting mutagenic nucleotide incorporation into RNA. mutT mutants are known to exhibit phenotypes independent of DNA mutational effects, which have been attributed to a decrease in transcription fidelity. The persistence of oxidized ribonucleotides in the available nucleotide pool and the subsequent incorporation of 8-oxo-GTP into mRNA, causing T to G transversions in the nascent transcript, was assumed to be the mechanism for RNA errors and these phenotypes. It was suggested that the absence of MutT can increase the readthrough of a stop codon mutation through 8-oxo-GTP incorporation generating a 30-fold increase in functional protein levels in mutT cultures compared to wild-type cultures. Such RNA infidelity in mutT strains may account for the accumulation of misfolded proteins and the observed cytotoxicity of aminoglycoside antibiotics. mutT phenotypes, such as protein mistranslation or antibiotic sensitivity, that have been attributed to 8-oxo-GTP require other explanations than simply 8-oxo-GTP misincorporation into mRNA.There are currently no reviews for this product.
KEGG: ecj:JW0097
STRING: 316385.ECDH10B_0081