Greater than 90% as determined by SDS-PAGE.
bla; men1Beta-lactamase CTX-M-1; EC 126.96.36.199; Beta-lactamase MEN-1; Cefotaximase 1
Target Protein Sequence
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Full Length of Mature Protein
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
The recombinant Escherichia coli Beta-lactamase CTX-M-1 (bla) was produced as a fusion protein with N-terminal 6xHis tagged in E.coli. The encoding sequence contains 29-291aa of Escherichia coli bla protein. This full-length of mature bla protein reached up to 90% in purity, which is measured by SDS-PAGE. It migrated to a molecular mass band of 32 kDa on the gel. It was also validated by the LC-MS/MS analysis. In-stock bla proteins are offered now. This recombinant bla protein may find uses in the specific antibody synthesis or the studies of microbiology.
blaCTX-M-1 is the most frequently detected extended-spectrum β-lactamases (ESBL)-encoding gene. Its encoding protein is a broad spectrum beta-lactamase that confers resistance to penicillins, as well as first, second, and third-generation cephalosporins.