Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
csrA; ECDH10B_2864; Translational regulator CsrA; Carbon storage regulator
Species
Escherichia coli (strain K12 / DH10B)
Target Protein Sequence
MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHREEIYQRIQAEKSQQSSY
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant E.coli csrA protein is encoded by the gene of csrA (1-61aa). The gene of csrA was cloned in a system (E.coli) that supported the expression of csrA and translation of messenger RNA. Modification of csrA by recombinant DNA technology could lead to the expression of the target protein. The purity is 85% determined by SDS-PAGE.
CsrA is a protein coding gene that encodes Translational regulator CsrA. According to some research, CsrA may have the following features.
Global regulation of small RNA binding protein CsrA and non-coding RNA molecule CsrB. Post-transcriptional global regulation of CsrA in bacteria. Under the influence of Escherichia coli global regulator CsrA, the formation and spread of biofilms. The RNA molecule CsrB binds to the global regulatory protein CsrA and antagonizes its activity in E. coli. CsrA regulates the translation of the carbon starvation gene cstA in E. coli by blocking ribosome access to cstA transcripts. The regulatory loops of E. coli CsrA/CsrB and BarA/UvrY systems. Identification and molecular characteristics of the polymorphic gene csrA in Escherichia coli, which affects glycogen biosynthesis, glucose production, cell size and surface characteristics.