Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
recF; uvrF; b3700; JW3677; DNA replication and repair protein RecF
Species
Escherichia coli (strain K12)
Expression Region
2-357aa
Target Protein Sequence
SLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIYTLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDGHKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQRNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTFSFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKLLMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISAEHVIDMSDENSKMFTVEKGKITD
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 2-357 form the expressed segment for recombinant Escherichia coli (strain K12) recF. The theoretical molecular weight of the recF protein is 56.4 kDa. Expression of this recF protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of recF, which enables a simple process of detecting and purifying the recF recombinant protein in the following steps.
The Escherichia coli DNA replication and repair protein RecF plays a crucial role in maintaining genomic integrity by participating in various aspects of DNA metabolism. RecF is involved in the restart of stalled DNA replication forks and the repair of DNA damage, particularly in situations where single-stranded DNA regions are generated. It forms a complex with RecR and RecO, collectively known as the RecFOR complex, which mediates the loading of RecA onto single-stranded DNA. RecA, in turn, promotes the strand exchange between single-stranded and double-stranded DNA, facilitating repair processes such as homologous recombination. RecF is essential for the cell's ability to respond to DNA damage, ensuring accurate and faithful DNA repair and replication. Understanding the functions of RecF is fundamental for unraveling the intricacies of bacterial DNA repair mechanisms and has implications for the development of antibacterial strategies targeting essential cellular processes.
