Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

F17a-A

Uniprot No.

P11312

Research Area

Microbiology

Alternative Names

F17a-AF17 fimbrial protein; F17 fimbrial adhesive antigen; F17 pilin

Species

Escherichia coli

Source

E.coli

Expression Region

22-180aa

Target Protein Sequence

YDGKITFNGKVVDQTCSVTTESKNLTVKLPTVSANSLASSGKVVGLTPFTILLEGCNTPAVTGAQNVNAYFEPNANTDYTTGNLTNTASSGASNVQIQLLNADGVKAIKLGQAAAAQSVDTVAINDANVTLRYNAQYYATGVATAGDVTSTVNYTIAYQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

32.4 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 22-180 constitute the expression domain of recombinant Escherichia coli F17a-A. The theoretical molecular weight of the F17a-A protein is 32.4 kDa. Expression of this F17a-A protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of F17a-A, which enables a simple process of detecting and purifying the F17a-A recombinant protein in the following steps.

The Escherichia coli F17 fimbrial protein (F17a-A) is a structural component of the F17 fimbriae, which are hair-like appendages on the surface of certain pathogenic E. coli strains. These fimbriae play a crucial role in the adhesion of bacteria to host cells, facilitating colonization and the establishment of infection. F17a-A is involved in the assembly and structural integrity of these appendages. The adhesion of E. coli expressing F17 fimbriae to host tissues is a key step in the pathogenesis of infections, particularly those affecting the intestines of animals. Understanding the structure and function of F17a-A can contribute to insights into bacterial virulence mechanisms and may inform the development of strategies to prevent or treat infections caused by F17-positive E. coli strains.

Target Background

Function

Fimbriae (also called pili), polar filaments radiating from the surface of the bacterium to a length of 0.5-1.5 micrometers and numbering 100-300 per cell, enable bacteria to colonize the epithelium of specific host organs.

Subcellular Location

Fimbrium.

Protein Families

Fimbrial protein family

Code	CSB-EP318126ENL
Abbreviation	Recombinant E.coli F17a-A protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP318126ENL could indicate that this peptide derived from E.coli-expressed Escherichia coli F17a-A. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP318126ENL could indicate that this peptide derived from E.coli-expressed Escherichia coli F17a-A.
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Recombinant Escherichia coli F17 fimbrial protein (F17a-A)

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