Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Microbiology
Alternative Names
F17a-AF17 fimbrial protein; F17 fimbrial adhesive antigen; F17 pilin
Expression Region
22-180aa
Target Protein Sequence
YDGKITFNGKVVDQTCSVTTESKNLTVKLPTVSANSLASSGKVVGLTPFTILLEGCNTPAVTGAQNVNAYFEPNANTDYTTGNLTNTASSGASNVQIQLLNADGVKAIKLGQAAAAQSVDTVAINDANVTLRYNAQYYATGVATAGDVTSTVNYTIAYQ
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 22-180 constitute the expression domain of recombinant Escherichia coli F17a-A. The theoretical molecular weight of the F17a-A protein is 32.4 kDa. Expression of this F17a-A protein is conducted in e.coli. The N-terminal 6xHis-SUMO tag was smoothly integrated into the coding gene of F17a-A, which enables a simple process of detecting and purifying the F17a-A recombinant protein in the following steps.
The Escherichia coli F17 fimbrial protein (F17a-A) is a structural component of the F17 fimbriae, which are hair-like appendages on the surface of certain pathogenic E. coli strains. These fimbriae play a crucial role in the adhesion of bacteria to host cells, facilitating colonization and the establishment of infection. F17a-A is involved in the assembly and structural integrity of these appendages. The adhesion of E. coli expressing F17 fimbriae to host tissues is a key step in the pathogenesis of infections, particularly those affecting the intestines of animals. Understanding the structure and function of F17a-A can contribute to insights into bacterial virulence mechanisms and may inform the development of strategies to prevent or treat infections caused by F17-positive E. coli strains.
