Product Details

Purity

>85% (SDS-PAGE)

Target Names

ihfA

Uniprot No.

P0A6X7

Alternative Names

ihfA; hid; himA; b1712; JW1702Integration host factor subunit alpha; IHF-alpha

Species

Escherichia coli (strain K12)

Expression Region

2-99

Target Protein Sequence

ALTKAEMSE YLFDKLGLSK RDAKELVELF FEEIRRALEN GEQVKLSGFG NFDLRDKNQR PGRNPKTGED IPITARRVVT FRPGQKLKSR VENASPKDE

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Related Products

Target Background

Function

One of the 2 subunits of integration host factor (IHF), a specific DNA-binding protein that functions in genetic recombination as well as in transcriptional and translational control. Binds to hundreds of transcriptionally inactive, AT-rich DNA sites, approximately half its binding sites are in non-coding DNA, which only accounts for about 10% of the genome.; Plays a crucial role in the lysogenic life cycle of bacteriophage lambda, as it is required not only in the recombination reaction, which inserts lambda DNA into the E.coli chromosome, but also for the synthesis of int and cI repressor, two phage proteins necessary for DNA insertion and repression, respectively. The synthesis of int and cI proteins is regulated indirectly by IHF via translational control of the lambda cII protein.; Has an essential role in conjugative DNA transfer (CDT), the unidirectional transfer of ssDNA plasmid from a donor to a recipient cell. It is the central mechanism by which antibiotic resistance and virulence factors are propagated in bacterial populations. Part of the relaxosome, which facilitates a site- and strand-specific cut in the origin of transfer by TraI, at the nic site. Relaxosome formation requires binding of IHF and TraY to the oriT region, which then facilitates binding of TraI.

Gene References into Functions

Cas1-Cas2-mediated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISPR leader sequences during spacer acquisition and immunologic memory. PMID: 27211867
Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system. PMID: 27899566
Data show that environment dependent selection between integration host factors (IHF) and DNA-binding proteins from starved cells (Dps) results in different physical organizations of DNA. PMID: 26657062
These results indicate that by combined transcriptional and translational controls of gene expression, Integration host factor activates expression of a specific set of genes required for survival at extremely acidic pH. PMID: 24816374
Authors found that, although oligomers were assembled in the absence of any individual high-affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential. PMID: 24443848
EcpR-mediated activation is aided by integration host factor (IHF), which is essential for counteracting the repression exerted by histone-like nucleoid-structuring protein (H-NS) on the ecp promoter. PMID: 22797761
By performing ChIP-seq experiments of each subunit of IHF in the absence of the other subunit, genome-wide maps of DNA binding of the proteins in their hetero- and homodimeric forms were defined. PMID: 22180530
analysis of the role of DNA deformation energy in sequence-specific DNA binding by Escherichia coli integration host factor PMID: 17035240
In the absence of TrwA, binding of integration host factor (IHF) to the oriT keeps the recombination levels low PMID: 17921309
A common function of IHF and HU in bacterial cells is to facilitate DNA organization in the nucleoid by the introduction of sharp bends in chromosomal DNA. PMID: 19132923
Activity of TraI was enhanced by the relaxosome components TraM and integration host factor(ihfA and ihfB). The magnitude of stimulation depended on the proximity of the specific protein binding sites to the position of open DNA. PMID: 19767439

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Subcellular Location

Cytoplasm, nucleoid.

Protein Families

Bacterial histone-like protein family

Database Links

KEGG: ecj:JW1702

STRING: 316385.ECDH10B_1848

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Recombinant Escherichia coli Integration host factor subunit alpha (ihfA)

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