Recombinant Escherichia coli Large-conductance mechanosensitive channel (mscL), partial

1. Because MscL opens to tension in the lipid bilayer, requiring no other proteins or ligands, it could be developed into a general noninvasive sonogenetic tool to manipulate the activities of neurons or other cells and potential nanodevices. PMID: 29916253
2. the amphipathic N-terminal helix of MscL acts as a crucial structural element during tension-induced gating, both stabilizing the closed state and coupling the channel to the membrane PMID: 27329693
3. More importantly, the FE model indicates that to fully operate an MscL nanovalve by pulling directly on the N-terminus and bottom of TM1, gain-of-function (GOF) mutants (e.g., G22N MscL) would have to be employed rather than the WT MscL channel. PMID: 28859093
4. Data suggest that dihydrostreptomycin binds to a specific site on mechanosensitive channel of large conductance (MscL) and modifies its conformation, thus allowing the passage of K+ and glutamate out of, and dihydrostreptomycin into, the cell. PMID: 27280286
5. comprehensive screen has identified many residues that are critical for channel function as well as highlighted MscL domains and residues that undergo the most drastic environmental changes upon gating. PMID: 26368283
6. Observe directly that the MscL channel opens via the helix-tilt model and the open pore reaches 2.8 nm in diameter. PMID: 24550255
7. The crystal structure of the cytoplasmic domain of MscL contains canonical hydrophobic and atypical ionic interactions compared to previously characterized coiled coil structures. PMID: 24038743
8. Results demonstrate the existence of oligomeric forms of MscL not yet observed by X-ray crystallography or other techniques and that in some cases a single type of MscL subunit can assemble as a mixture of oligomeric states. PMID: 21280123
9. Data show that by using planar membrane bilayers MscL forms functional ion channels capable of ion transport. PMID: 21134371
10. MscL does not need the insertase YidC for insertion in vitro PMID: 20888317
11. Data show that introduction of a charge into pore lumen of MscL does indeed influence the permeation of charged molecules. PMID: 20930114
12. the distribution of channels among the subconducting states of MscL is significantly less dependent on tension than the distribution between the closed and conducting states PMID: 15111402
13. lipid effects altered activity through examined the contribution of specific headgroups by measuring their effect on the membrane tension required to gate the MscL channel PMID: 16142922
14. Tyrosines and tryptophans that anchor both ends of the helices to membrane interfaces in many transmembrane proteins are not common in MscL and homologous mechanosensitive channels. PMID: 16156671
15. YidC is essential for efficient membrane insertion of the MscL protein PMID: 17113597
16. Pharmacological potential of MscL may involve discovery of new age antibiotics to combat multiple drug-resistant bacterial strains. PMID: 17350877
17. model for structural transitions and residue-residue proximities that occur upon MscL gating. PMID: 19261722

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KEGG: ecj:JW3252

STRING: 316385.ECDH10B_3465