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Recombinant Escherichia coli Low molecular weight protein-tyrosine-phosphatase wzb (wzb)

Recombinant Escherichia coli Low molecular weight protein-tyrosine-phosphatase wzb (wzb)

Code	CSB-YP364465ENV
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP364465ENV
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP364465ENV-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP364465ENV
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP364465ENV
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

wzb

Uniprot No.

P0AAB2

Alternative Names

wzb; b2061; JW2046; Low molecular weight protein-tyrosine-phosphatase Wzb; EC 3.1.3.48

Species

Escherichia coli (strain K12)

Expression Region

1-147

Target Protein Sequence

MFNNILVVCV GNICRSPTAE RLLQRYHPEL KVESAGLGAL VGKGADPTAI SVAAEHQLSL EGHCARQISR RLCRNYDLIL TMEKRHIERL CEMAPEMRGK VMLFGHWDNE CEIPDPYRKS RETFAAVYTL LERSARQWAQ ALNAEQV

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Dephosphorylates Wzc. Required for the extracellular polysaccharide colanic acid synthesis, probably involved in the export of colanic acid from the cell to medium. Involved in protection of cells against contact-dependent growth inhibition (CDI), probably due to the loss of a physical impediment to cell-cell contact.

Gene References into Functions

Escherichia coli Wzb solution structure reveals a novel substrate recognition mechanism of prokaryotic low molecular weight protein-tyrosine phosphatases PMID: 16651264
Results present the structure of Wzb in complex with phosphate and high-resolution structures of apo-CpsB and a phosphate-complexed CpsB. PMID: 19616007

Protein Families

Low molecular weight phosphotyrosine protein phosphatase family

Database Links

KEGG: ecj:JW2046

STRING: 316385.ECDH10B_2211

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