|Have Questions?||Leave a Message or Start an on-line Chat|
There are currently no reviews for this product.
I tested this protein in a functional assay, and found that it had low-levels of activity. Apparently, metal ions are necessary for the enzymatic function of this protein, and they were curious to know what metal ions (Zn ions, Fe ions, and Ni ions), if any, were specifically added to the culture medium. Could you please advise on which ones and at what concentrations?
Also, I was wondering if you had the ability to increase the concentration of metal ions, specifically 20 mM NiCl2, throughout the expression/purification process, to try and achieve higher activity. Would this be something your lab might be able to do for the customer?
I am fine with ordering the protein again, just as long as I can confirm to them that I can provide them with higher concentrations of metal ions during the expression/purification process. I also provided the article , which explains how to increase the metal ion concentrations to achieve higher activity.
Can you please let me know if any of this would be possible?