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Recombinant Escherichia coli Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)

Recombinant Escherichia coli Phospho-2-dehydro-3-deoxyheptonate aldolase, Trp-sensitive (aroH)

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Citations (1)

Code	CSB-EP355572ENV
Abbreviation	Recombinant E.coli aroH protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

aroH

Uniprot No.

P00887

Research Area

others

Alternative Names

3-deoxy-D-arabino-heptulosonate 7-phosphate synthase DAHP synthase Phospho-2-keto-3-deoxyheptonate aldolase

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

1-348aa

Target Protein Sequence

MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEKILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVGWKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGAIGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMTIYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRRQLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGSQPLTYGQSITDPCLGWEDTERLVEKLASAVDTRF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

42.8 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

To achieve the expression of the recombinant Escherichia coli (strain K12) aroH protein in e.coli cells, a DNA fragment encoding the Escherichia coli (strain K12) aroH protein (1-348aa) is inserted into a plasmid vector, which is then transferred to e.coli cells. After screening positive cells, they are cultured and induced to creat the aroH protein. The protein features a N-terminal 6xHis tag fusion. The recombinant Escherichia coli (strain K12) aroH protein is purified from the cell lysate through affinity purification. The purity of the obtained recombinant Escherichia coli (strain K12) aroH protein is greater than 85%, measured by SDS-PAGE.

Citations

Revealing novel protein interaction partners of glyphosate in Escherichia coli AS Aldehoff, D Türkowsky, P Lohmann,Environment International,2024

Customer Reviews and Q&A

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Target Background

Function

Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP).

Protein Families

Class-I DAHP synthase family

Database Links

KEGG: ecj:JW1694

STRING: 316385.ECDH10B_1840

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