Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
serB; b4388; JW4351; Phosphoserine phosphatase; PSP; PSPase; EC 3.1.3.3; O-phosphoserine phosphohydrolase
Species
Escherichia coli (strain K12)
Expression Region
1-322aa
Target Protein Sequence
MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYGRGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLGKIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRSRVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKLRLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGANDLPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
This Escherichia coli serB recombinant protein was produced in E.coli, where the gene sequence encoding Escherichia coli serB (1-322aa) was expressed with the N-terminal 6xHis-SUMO tag. The purity of this serB protein was greater than 90% by SDS-PAGE.
SerB encodes the SerB enzyme. In E. coli and other bacteria, the expression of the serB gene is regulated by various factors to ensure the synthesis of an adequate amount of serine under appropriate conditions. SerB is one of the key enzymes in the serine biosynthesis pathway. Serine is an essential amino acid crucial for the survival of organisms as it is one of the building blocks for protein synthesis. The role of the SerB enzyme is to hydrolyze the product of serine phosphorylation (3-phosphoserine) into serine, thereby providing the organism with the required serine. SerB enzyme also has importance in the field of genetic engineering. It can be used to regulate the serine biosynthesis pathway, thereby altering the serine synthesis capability of bacteria or other microorganisms.
