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Recombinant Escherichia coli Protein yebF (yebF)

Recombinant Escherichia coli Protein yebF (yebF)

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Code	CSB-EP329240ENV
Abbreviation	Recombinant E.coli yebF protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

yebF

Uniprot No.

P33219

Research Area

others

Alternative Names

yebF; b1847; JW1836; Protein YebF

Species

Escherichia coli (strain K12)

Source

E.coli

Expression Region

22-118aa

Target Protein Sequence

ANNETSKSVTFPKCEDLDAAGIAASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHYQVSVDCKAGMAEYQRR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

15.8kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 22-118 constitute the expression domain of recombinant Escherichia coli (strain K12) yebF. The calculated molecular weight for this yebF protein is 15.8 kDa. This protein is generated in a e.coli-based system. The N-terminal 10xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of yebF, which enables a simple process of detecting and purifying the yebF recombinant protein in the following steps.

The Escherichia coli protein YebF is a secreted peptide that serves as a carrier for transgenic proteins, offering a solution to circumvent toxicity and contamination concerns related to protein generation in E. coli. Studies have shown that YebF is an effective fusion partner that carries recombinant target proteins from the cytosol of E. coli into the medium.