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Recombinant Helicobacter pylori Urease subunit beta (ureB)

Recombinant Helicobacter pylori Urease subunit beta (ureB)

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Code	CSB-YP300910HUV
MSDS	MSDS Datasheet
Size	US$88
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP300910HUV could indicate that this peptide derived from Yeast-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) ureB. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP300910HUV could indicate that this peptide derived from Yeast-expressed Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori) ureB.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ureB

Uniprot No.

P69996

Alternative Names

HP_0072; hpuB; URE1_HELPY; Urea amidohydrolase subunit beta; Urease subunit beta; ureB

Species

Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)

Source

Yeast

Expression Region

1-569aa

Target Protein Sequence

MKKISRKEYVSMYGPTTGDKVRLGDTDLIAEVEHDYTIYGEELKFGGGKTLREGMSQSNNPSKEELDLIITNALIVDYTGIYKADIGIKDGKIAGIGKGGNKDMQDGVKNNLSVGPATEALAGEGLIVTAGGIDTHIHFISPQQIPTAFASGVTTMIGGGTGPADGTNATTITPGRRNLKWMLRAAEEYSMNLGFLAKGNASNDASLADQIEAGAIGFKIHEDWGTTPSAINHALDVADKYDVQVAIHTDTLNEAGCVEDTMAAIAGRTMHTFHTEGAGGGHAPDIIKVAGEHNILPASTNPTIPFTVNTEAEHMDMLMVCHHLDKSIKEDVQFADSRIRPQTIAAEDTLHDMGIFSITSSDSQAMGRVGEVITRTWQTADKNKKEFGRLKEEKGDNDNFRIKRYLSKYTINPAIAHGISEYVGSVEVGKVADLVLWSPAFFGVKPNMIIKGGFIALSQMGDANASIPTPQPVYYREMFAHHGKAKYDANITFVSQAAYDKGIKEELGLERQVLPVKNCRNITKKDMQFNDTTAHIEVNPETYHVFVDGKEVTSKPANKVSLAQLFSIF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

63.7kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

A full-length Helicobacter pylori Urease subunit beta (ureB) ORF (1-569aa) with an N-terminal 6xHis-tag was expressed in the yeast. The forming protein is the Recombinant Helicobacter pylori ureB protein. This ureB protein was verified by SDS-PAGE and LC-MS/MS Analyses. Its purity is greater than 90%. It has a calculated molecular mass of 63.7 kDa. This recombinant ureB protein may be used as an immunogen for specific antibody production or in the studies of gastritis and gastric cancer.

UreB is a structural subunit of urease that is critical to the metabolism and virulence of Helicobacter pylori. Urease is also necessary for Helicobacter pylori's colonization of the gastric mucosa and is a potent immunogen that triggers a vigorous immune response. Chronic Helicobacter pylori (H. pylori) infection to gastric mucosa is a common cause for gastritis and increases the risk of gastric cancer.