Recombinant Horse Fibrinogen beta chain (FGB)

In Stock
Code CSB-EP4095HO
MSDS
Size $554
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Cardiovascular
Species
Equus caballus (Horse)
Source
E.coli
Expression Region
28-495aa
Target Protein Sequence
QALDYDHEEEDGRTKVTFDARSHRPLDKKREEPLSLKPAPPPISGPGYRPRPAKVAANKKKVERKAPDAGGCVHADPDLGVLCPTGCQLQDTLVKQERSVRNSVNELSHNVESISQSSSSNFQYITALKEMWKKREKQLKDNENVVGEYSSELEKHQLYIDETVNSNVPTNIRVLRSILENLRSKIQKLESDVSAQMEYCRNPCTVSCNIPVVSGRECEEVIRNGGETSEMYLIQPDRSAKPYRVYCDMTTESGGWTVIQNRQDGSVDFGRKWDPYKQGFGNIATNADGKKYCGLPGEYWLGNDKISQLTMMGPTVLLIEMEDWRGDKVKALYEGFTVKGEGDKYQLSVGKYKGTAGNALVDGASQLVGENRTMTIHNGMYFSTYDRDNDGWTTTDPRKQCSKEDGGGWWYNRCHAANPNGRYYWGGQYSWDMAKHGTDDGVVWMNWKGSWYSMKKISMKIRPYFPQQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
53.2 kDa
Protein Length
Full Length of Mature Protein
Tag Info
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

In the process of producing the recombinant Equus caballus (Horse) FGB protein, e.coli cells are transfected with a DNA expression vector containing the gene for the desired protein (28-495aa). Subsequent culturing induces the expression of the intended protein. The recombinant Equus caballus (Horse) FGB protein is then collected and purified from the cell lysate through affinity purification, displaying a purity exceeding 85%, as verified by SDS-PAGE.

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