Recombinant Human 28S ribosomal protein S27, mitochondrial (MRPS27)

Code CSB-YP842129HU
MSDS
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Source Yeast
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Code CSB-EP842129HU
MSDS
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Source E.coli
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Code CSB-EP842129HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842129HU
MSDS
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Source Baculovirus
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Code CSB-MP842129HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MRPS27
Uniprot No.
Alternative Names
28S ribosomal protein S27; 28S ribosomal protein S27, mitochondrial; KIAA0264; Mitochondrial 28S ribosomal protein S27; mitochondrial; mitochondrial ribosomal protein S27; MRP-S27; MRPS27; RT27_HUMAN; S27mt
Species
Homo sapiens (Human)
Expression Region
1-414
Target Protein Sequence
MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAEY YLYKFRHSPN CWYLRNWTIH TWIRQCLKYD AQDKALYTLV NKVQYGIFPD NFTFNLLMDS FIKKENYKDA LSVVFEVMMQ EAFEVPSTQL LSLYVLFHCL AKKTDFSWEE ERNFGASLLL PGLKQKNSVG FSSQLYGYAL LGKVELQQGL RAVYHNMPLI WKPGYLDRAL QVMEKVAASP EDIKLCREAL DVLGAVLKAL TSADGASEEQ SQNDEDNQGS EKLVEQLDIE ETEQSKLPQY LERFKALHSK LQALGKIESE GLLSLTTQLV KEKLSTCEAE DIATYEQNLQ QWHLDLVQLI QREQQQREQA KQEYQAQKAA KASA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding component of the mitochondrial small ribosomal subunit (mt-SSU) that plays a role in mitochondrial protein synthesis. Stimulates mitochondrial mRNA translation of subunit components of the mitochondrial electron transport chain. Binds to the mitochondrial 12S rRNA (12S mt-rRNA) and tRNA(Glu). Involved also in positive regulation of cell proliferation and tumor cell growth.
Gene References into Functions
  1. we verified for the first time that mitochondrial ribosomal protein S23 expression was upregulated in hepatocellular carcinoma. High mitochondrial ribosomal protein S23 levels can predict poor clinical outcomes in hepatocellular carcinoma, and this protein plays a key role in tumor proliferation. Therefore, mitochondrial ribosomal protein S23 may be a potential therapeutic target for hepatocellular carcinoma. PMID: 28714366
  2. MRPS27 is required for mitochondrial protein synthesis PMID: 22841715
Subcellular Location
Cytoplasm. Mitochondrion.
Protein Families
Mitochondrion-specific ribosomal protein mS27 family
Tissue Specificity
Overexpressed in hepatocellular carcinoma tissues compared with adjacent non-tumoral liver tissues (at protein level). Ubiquitous.
Database Links

HGNC: 14512

OMIM: 611989

KEGG: hsa:23107

STRING: 9606.ENSP00000261413

UniGene: Hs.482491

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