Recombinant Human A-kinase-interacting protein 1 (AKIP1)

Code CSB-YP868282HU
MSDS
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Source Yeast
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Code CSB-EP868282HU
MSDS
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Source E.coli
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Code CSB-EP868282HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868282HU
MSDS
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Source Baculovirus
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Code CSB-MP868282HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AKIP1
Uniprot No.
Alternative Names
AKIP1; BCA3; C11orf17A-kinase-interacting protein 1; Breast cancer-associated gene 3 protein; PKA-interacting protein; Proline-rich protein BCA3
Species
Homo sapiens (Human)
Expression Region
1-210
Target Protein Sequence
MDNCLAAAAL NGVDRRSLQR SARLALEVLE RAKRRAVDWH ALERPKGCMG VLAREAPHLE KQPAAGPQRV LPGEREERPP TLSASFRTMA EFMDYTSSQC GKYYSSVPEE GGATHVYRYH RGESKLHMCL DIGNGQRKDR KKTSLGPGGS YQISEHAPEA SQPAENISKD LYIEVYPGTY SVTVGSNDLT KKTHVVAVDS GQSVDLVFPV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Enhances NF-kappa-B transcriptional activity by regulating the nuclear localization of the NF-kappa-B subunit RELA and promoting the phosphorylation of RELA by PRKACA. Regulates the effect of the cAMP-dependent protein kinase signaling pathway on the NF-kappa-B activation cascade.
Gene References into Functions
  1. These results show that AKIP1 is crucial in cervical cancer angiogenesis and growth by elevating the levels of the NF-kappaB-dependent chemokines CXCL1, CXCL2, and CXCL8. PMID: 29520695
  2. Breast carcinoma-associated protein (BCA3) is packaged into Human immunodeficiency virus 1 (HIV-1) particles, and the BCA3 C-terminus is responsible for this incorporation. BCA3 incorporation into HIV-1 particles is mediated by its ability to interact with the catalytic subunit of protein kinase A. There is no effect of incorporated BCA3 on HIV-1 infectivity. PMID: 29677171
  3. The pleiotropic effects of BCA3 in HCC progression. PMID: 29133128
  4. The absence of exon 3 and exon 5 influenced the subcellular localization of BCA3 and nuclear factor kappa B (NF-kB)-dependent gene expression. PMID: 25526186
  5. Data indicate an important role for A-kinase-interacting protein 1 (AKIP1) in esophageal squamous cell carcinoma (ESCC) angiogenesis and lymphangiogenesis. PMID: 24413079
  6. Expression of BCA3 did not affect Mason-Pfizer money virus (MPMV) particle release and proteolytic processing; however, it slightly increased MPMV infectivity. PMID: 24659101
  7. Data indicate A-kinase interacting protein 1 (AKIP1) as a critical molecular regulator and a therapeutic control point for stress adaptation in the heart. PMID: 23319652
  8. action of PKA signaling on the NF-kappaB activation cascade PMID: 20562110
  9. characterization of the BCA3 gene; BCA3 is expressed in breast tumor cells in vivo, and not in surrounding stromal tissue PMID: 12527432
  10. AKIP1 describes a PKA-interacting protein that can contribute to localization by a mechanism that is distinct from A-kinase anchoring proteins that interact with the regulatory subunits. PMID: 15630084
  11. AKIP1 colocalized with p65 within the cells and appeared to retain p65 in nucleus. PMID: 18178962

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Subcellular Location
Nucleus. Note=Locates to punctate spots.
Tissue Specificity
Expressed at high levels in adult heart and at lower levels in brain, testis, ovary and skeletal muscle. Up-regulated in some breast cancer cell lines. Isoform 1 and isoform 3 are expressed in fetal brain.
Database Links

HGNC: 1170

OMIM: 609191

KEGG: hsa:56672

STRING: 9606.ENSP00000310459

UniGene: Hs.131180

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