Recombinant Human AF4/FMR2 family member 2 (AFF2), partial

Code CSB-YP001413HU
MSDS
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Source Yeast
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Code CSB-EP001413HU
MSDS
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Source E.coli
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Code CSB-EP001413HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001413HU
MSDS
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Source Baculovirus
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Code CSB-MP001413HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AFF2
Uniprot No.
Alternative Names
AF4/FMR2 family member 2; AF4/FMR2 family, member 2; AFF2; AFF2_HUMAN; FMR2; FMR2P; Fragile X E mental retardation syndrome protein; fragile X mental retardation 2; Fragile X mental retardation 2 protein; fragile X mental retardation gene associated with FRAXE; FRAXE; mild or borderline mental retardation; MRX2; OTTHUMP00000024204; OX19; Protein FMR-2; Protein Ox19
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
RNA-binding protein. Might be involved in alternative splicing regulation through an interaction with G-quartet RNA structure.
Gene References into Functions
  1. Partial AFF2 microduplication in a patient with auditory processing disorder, emotional impairment and macrosomia. PMID: 25256661
  2. FMR2 is an upstream regulator of FOS and JUN, and further link deregulation of the immediate early response genes to the pathology of ID- and FRAXE-associated ID in particular. PMID: 23562910
  3. 2.5% of males Autism spectrum disorder patients had missense mutations in AFF2 at highly conserved evolutionary sites. PMID: 22773736
  4. A report of novel deletions involving AFF2 provide evidence for a new mutational spectrum, microdeletions, that are responsible for Fragile X E in a small subset of patients. PMID: 22065534
  5. overexpression of AFF2/3/4 interferes with the organization and/or biogenesis of nuclear speckles. PMID: 21330300

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Involvement in disease
Mental retardation, X-linked, associated with fragile site FRAXE (MRFRAXE)
Subcellular Location
Nucleus speckle. Note=When splicing is inhibited, accumulates in enlarged speckles.
Protein Families
AF4 family
Tissue Specificity
Brain (most abundant in hippocampus and amygdala), placenta and lung.
Database Links

HGNC: 3776

OMIM: 300806

KEGG: hsa:2334

STRING: 9606.ENSP00000359489

UniGene: Hs.496911

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