Recombinant Human AMSH-like protease (STAMBPL1)

Code CSB-YP853417HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP853417HU
MSDS
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Source E.coli
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Code CSB-EP853417HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP853417HU
MSDS
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Source Baculovirus
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Code CSB-MP853417HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
STAMBPL1
Uniprot No.
Alternative Names
ALMalpha; AMSH FP; AMSH LP; AMSH-like protease; AMSH-LP; AMSHFP; AMSHLP; Associated molecule with the SH3 domain of STAM (AMSH) like protein; FLJ31524; OTTHUMP00000020055; STALP_HUMAN; STAM binding protein like 1; STAM-binding protein-like 1; STAMBPL1
Species
Homo sapiens (Human)
Expression Region
1-436
Target Protein Sequence
MDQPFTVNSL KKLAAMPDHT DVSLSPEERV RALSKLGCNI TISEDITPRR YFRSGVEMER MASVYLEEGN LENAFVLYNK FITLFVEKLP NHRDYQQCAV PEKQDIMKKL KEIAFPRTDE LKNDLLKKYN VEYQEYLQSK NKYKAEILKK LEHQRLIEAE RKRIAQMRQQ QLESEQFLFF EDQLKKQELA RGQMRSQQTS GLSEQIDGSA LSCFSTHQNN SLLNVFADQP NKSDATNYAS HSPPVNRALT PAATLSAVQN LVVEGLRCVV LPEDLCHKFL QLAESNTVRG IETCGILCGK LTHNEFTITH VIVPKQSAGP DYCDMENVEE LFNVQDQHDL LTLGWIHTHP TQTAFLSSVD LHTHCSYQLM LPEAIAIVCS PKHKDTGIFR LTNAGMLEVS ACKKKGFHPH TKEPRLFSIC KHVLVKDIKI IVLDLR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Zinc metalloprotease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Does not cleave 'Lys-48'-linked polyubiquitin chains.
Gene References into Functions
  1. Catalytic Mechanism of Human AMSH-LP Domain Deubiquitinating Enzymes PMID: 26256234
  2. Cellular STAMBPL1 is a positive regulator of human T-cell leukemia virus type 1 Tax-mediated NF-kappaB signaling. PMID: 22258247
  3. Data show that associated molecule with the SH3 domain of STAM-like protein (AMSH-LP), unlike AMSH, fails to bind to the SH3 domains of STAM1 (signal transducing adaptor molecule 1) and Grb2. PMID: 12810066
  4. AMSH and AMSH-LP are anchored on the early endosomal membrane via interaction with the clathrin coat PMID: 16716190
  5. AMSH redirects CaR from slow recycling to down-regulation, reducing CaR expression and decreasing PTHrP secretion. PMID: 17426287

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Protein Families
Peptidase M67C family
Tissue Specificity
Ubiquitously expressed.
Database Links

HGNC: 24105

OMIM: 612352

KEGG: hsa:57559

STRING: 9606.ENSP00000360992

UniGene: Hs.16229

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