Recombinant Human ATP-binding cassette sub-family F member 1 (ABCF1), partial

Code CSB-YP822770HU
MSDS
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Source Yeast
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Code CSB-EP822770HU
MSDS
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Source E.coli
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Code CSB-EP822770HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP822770HU
MSDS
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Source Baculovirus
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Code CSB-MP822770HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ABCF1
Uniprot No.
Alternative Names
ABC27; ABC50; ABCF1; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette; sub family F (GCN20); member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Gene References into Functions
  1. The high copy number variant in the ABCF1 gene was the strongest risk factor for gout. PMID: 28405828
  2. ABCF1 extrinsically regulates retinal pigment epithelial cell phagocytosis of shed photoreceptor outer segments. PMID: 25904329
  3. Elevated microRNA-23a Expression Enhances the Chemoresistance of Colorectal Cancer Cells with Microsatellite Instability to 5-Fluorouracil by Directly Targeting ABCF1 PMID: 25929864
  4. expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. PMID: 25597744
  5. A rare copy number variation of the ABCF1 gene was detected among dengue patients from Malaysia. PMID: 24634119
  6. The results suggested that SNPs at PRR3 and ABCF1 genes and the haplotype composed by SNPs at GNL1 and PRR3 between the HLA-A and HLA-C genes tended to predict Graves ophthalmology in a gender-dependent manner in patients with Graves disease in Taiwan. PMID: 24908204
  7. ABCF1 showed a significant increase in expression after escitalopram treatment. PMID: 23719290
  8. ABCF1 is a critical protein that associates with viral double-stranded DNA. PMID: 23263557
  9. The two critical HLA regions for susceptibility to autoimmune pancreatitis are limited to the HLA-DRB1*0405-DQB1*0401 in the class II and the ABCF1in the class I regions. PMID: 17119950
  10. ABC50 N-terminal region interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2 PMID: 17894550
  11. ABC50 plays a key role in translation initiation and has functions that are distinct from those of other non-membrane ABC proteins. PMID: 19570978

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Subcellular Location
[Isoform 2]: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.
Protein Families
ABC transporter superfamily, ABCF family, EF3 subfamily
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 70

OMIM: 603429

KEGG: hsa:23

STRING: 9606.ENSP00000313603

UniGene: Hs.655285

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