ABCF1 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA216952A0HU
Size	US$210
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Image	IHC image of CSB-RA216952A0HU diluted at 1:100 and staining in paraffin-embedded human stomach tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA216952A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of HeLa cell with CSB-RA216952A0HU at 1:50 , counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing Hela cells stained with CSB-RA216952A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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Target Background

Product Details

Uniprot No.

Q8NE71

Target Names

ABCF1

Alternative Names

ABC27 antibody; ABC50 antibody; ABCF1 antibody; ABCF1_HUMAN antibody; ATP binding cassette 50 (TNF alpha stimulated) antibody; ATP binding cassette 50 antibody; ATP binding cassette sub family F member 1 antibody; ATP binding cassette; sub family F (GCN20); member 1 antibody; ATP-binding cassette 50 antibody; ATP-binding cassette sub-family F member 1 antibody; AU041969 antibody; D17Wsu166e antibody; EST123147 antibody; GCN20 antibody; TNF alpha stimulated ABC protein antibody; TNF-alpha-stimulated ABC protein antibody; TNFalpha inducible ATP binding protein antibody; wu:fb79c06 antibody; wu:fc39a05 antibody; wu:fj94a08 antibody; zgc:85667 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human ABCF1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8G6

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

ABCF1, also known as ATP-binding cassette sub-family F member 1, plays a distinctive role among ABC transporter family members. Rather than functioning as a classical membrane transporter, ABCF1 operates as a cytoplasmic protein involved in translation initiation and immune signaling pathways. Its responsiveness to TNF-alpha stimulation has made it a protein of considerable interest in studies examining inflammatory responses and cellular stress mechanisms, positioning it as a relevant target for researchers investigating epigenetics and nuclear signaling processes.

This recombinant monoclonal antibody, clone 8G6, offers the reproducibility that demanding experimental workflows require. Developed using recombinant technology with a defined sequence, this rabbit IgG provides the lot-to-lot consistency essential for longitudinal studies and multi-site collaborations. The antibody was raised against a synthesized peptide derived from human ABCF1 and purified through affinity chromatography, ensuring high specificity for your target.

Validation across multiple platforms demonstrates genuine experimental flexibility. Immunohistochemistry studies in paraffin-embedded human stomach tissue and cervical cancer specimens show clear detection at 1:100 dilution using citrate buffer antigen retrieval. For cellular localization studies, immunofluorescence staining in HeLa cells reveals distinct signal patterns when counter-stained with DAPI. Flow cytometry validation, also performed in HeLa cells, confirms utility for quantitative single-cell analysis, with clear separation between specific staining and isotype control populations.

Whether you are characterizing ABCF1 expression in clinical tissue specimens, examining subcellular distribution in cultured cells, or quantifying protein levels across experimental conditions, this antibody provides a reliable tool for advancing your understanding of this multifunctional ABC family member.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.

Gene References into Functions

The high copy number variant in the ABCF1 gene was the strongest risk factor for gout. PMID: 28405828
ABCF1 extrinsically regulates retinal pigment epithelial cell phagocytosis of shed photoreceptor outer segments. PMID: 25904329
Elevated microRNA-23a Expression Enhances the Chemoresistance of Colorectal Cancer Cells with Microsatellite Instability to 5-Fluorouracil by Directly Targeting ABCF1 PMID: 25929864
expression of ABC50 mutants, which cannot hydrolyse ATP, decreases general translation and relaxes the discrimination against the use of non-AUG codons at translation start sites. PMID: 25597744
A rare copy number variation of the ABCF1 gene was detected among dengue patients from Malaysia. PMID: 24634119
The results suggested that SNPs at PRR3 and ABCF1 genes and the haplotype composed by SNPs at GNL1 and PRR3 between the HLA-A and HLA-C genes tended to predict Graves ophthalmology in a gender-dependent manner in patients with Graves disease in Taiwan. PMID: 24908204
ABCF1 showed a significant increase in expression after escitalopram treatment. PMID: 23719290
ABCF1 is a critical protein that associates with viral double-stranded DNA. PMID: 23263557
The two critical HLA regions for susceptibility to autoimmune pancreatitis are limited to the HLA-DRB1*0405-DQB1*0401 in the class II and the ABCF1in the class I regions. PMID: 17119950
ABC50 N-terminal region interacts with eukaryotic initiation factor eIF2 and is a target for regulatory phosphorylation by CK2 PMID: 17894550
ABC50 plays a key role in translation initiation and has functions that are distinct from those of other non-membrane ABC proteins. PMID: 19570978

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Subcellular Location

[Isoform 2]: Cytoplasm. Nucleus, nucleoplasm. Nucleus envelope.

Protein Families

ABC transporter superfamily, ABCF family, EF3 subfamily

Tissue Specificity

Ubiquitous.

Database Links

HGNC: 70

OMIM: 603429

KEGG: hsa:23

STRING: 9606.ENSP00000313603

UniGene: Hs.655285

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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