Recombinant Human Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (ST6GALNAC6), partial,Yeast

Code CSB-YP850251HU
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ST6GALNAC6
Uniprot No.
Alternative Names
ST6GALNAC6; SIAT7F; UNQ708/PRO1359; Alpha-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6; EC 2.4.99.-; GalNAc alpha-2,6-sialyltransferase VI; ST6GalNAc VI; ST6GalNAcVI; hST6GalNAc VI; Sialyltransferase 7F; SIAT7-F
Species
Homo sapiens (Human)
Source
Yeast
Protein Length
full length protein
Tag Info
N-terminal His-tagged
Tag-Free
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Transfers the sialyl group (N-acetyl-alpha-neuraminyl or NeuAc) from CMP-NeuAc onto glycoproteins and glycolipids, forming an alpha-2,6-linkage. Produces branched type disialyl structures by transfer of a sialyl group onto the GalNAc or GlcNAc residue inside backbone core chains having a terminal sialic acid with an alpha-2,3-linkage on Gal. ST6GalNAcVI prefers glycolipids to glycoproteins, predominantly catalyzing the biosynthesis of ganglioside GD1alpha from GM1b. Besides GMb1, MSGG and other glycolipids, it shows activity towards sialyl Lc4Cer generating disialyl Lc4Cer, which can lead to the synthesis of disialyl Lewis a (Le(a)), suggested to be a cancer-associated antigen. Also has activity toward GD1a and GT1b, and can generate DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside).
Gene References into Functions
  1. novel substrate specificity of ST6GalNAc VI, which is responsible for the synthesis of disialyl Lea but not for alpha-series gangliosides in human colon tissues PMID: 12668675
  2. ST6GalNAc-VI and disialylgalactosylgloboside were found in proximal tubule epithelial cells in normal kidney tissues PMID: 17123352
Subcellular Location
Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 29 family
Tissue Specificity
Expressed in kidney, in proximal tubule epithelial cells. Expressed in colon cell lines.
Database Links

HGNC: 23364

OMIM: 610135

KEGG: hsa:30815

STRING: 9606.ENSP00000291839

UniGene: Hs.109672

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