Recombinant Human Ammonium transporter Rh type B (RHBG), partial

Code CSB-YP887968HU
MSDS
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Source Yeast
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Code CSB-EP887968HU
MSDS
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Source E.coli
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Code CSB-EP887968HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887968HU
MSDS
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Source Baculovirus
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Code CSB-MP887968HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RHBG
Uniprot No.
Alternative Names
Ammonium transporter Rh type B; Rh family B glycoprotein; Rh family type B glycoprotein; Rh type B glycoprotein; RHBG; RHBG_HUMAN; Rhesus blood group B glycoprotein; Rhesus blood group family type B glycoprotein; SLC42A2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as a specific ammonium transporter.
Gene References into Functions
  1. RHBG expression as a direct target of beta-catenin regulation. PMID: 26029888
  2. Kidney AE1 actually associates with epithelial ankyrin-G and renal ammonium transporter RhBG, which also binds ankyrin-G. PMID: 25616663
  3. Rh B Glycoprotein (Rhbg) and Rh C Glycoprotein (Rhcg) are identified as ammonia transporters. They are expressed in specific cell populations and membrane domains in distal renal epithelial cells, where they facilitate ammonia secretion. PMID: 24647713
  4. RhAG, RhBG and RhCG-exhibit significant permeability to NH3 and show for the first time that RhBG and RhCG can conduct CO2. PMID: 24077989
  5. human kidney expresses basolateral RhBG protein in connecting segment, type A intercalated cells, and non-A, non-B cells PMID: 23324176
  6. Electric current measurements and intracellular pHi determinations suggest that RhBG acts as an electroneutral NH4+-H+ exchanger. PMID: 15284342
  7. RhBG requires a tyrosine-based signal and ankyrin-G for basolateral targeting and membrane PMID: 15611082
  8. RhBG and RhCG facilitate ammonium movement across the plasma membrane; these results favor the hypothesis that these Rh glycoproteins, together with their homolog RhAG, constitute a family of NH3 channels in mammalian cells. PMID: 15929723
  9. Phosphorylation and ankyrin-G binding of the C-terminal domain regulate targeting and function of the ammonium transporter RhBG PMID: 18635543
  10. This study concludes that under normal conditions, RhCG is the major putative ammonia transporter expressed in the human kidney and RhBG is not expressed at detectable levels. PMID: 19357182
  11. Rhbg and Rhcg may contribute to bronchial epithelial cell ammonia metabolism and suggest that they do not contribute to pulmonary carbon dioxide transport. PMID: 19429772

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Subcellular Location
Basolateral cell membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Protein Families
Ammonium transporter (TC 2.A.49) family, Rh subfamily
Tissue Specificity
Specifically expressed in kidney. Also detected in liver and ovary.
Database Links

HGNC: 14572

OMIM: 607079

KEGG: hsa:57127

STRING: 9606.ENSP00000357229

UniGene: Hs.131835

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