Recombinant Human Amphoterin-induced protein 2 (AMIGO2), partial

Code CSB-YP769765HU
MSDS
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Source Yeast
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Code CSB-EP769765HU
MSDS
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Source E.coli
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Code CSB-EP769765HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP769765HU
MSDS
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Source Baculovirus
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Code CSB-MP769765HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AMIGO2
Uniprot No.
Alternative Names
Adhesion molecule with Ig like domain 2; ALI 1; ALI1; Alivin 1; Alivin-1; Alivin1; AMGO2_HUMAN; AMIGO 2; AMIGO-2; Amigo2; Amphoterin induced gene 2; Amphoterin induced protein 2; Amphoterin-induced gene and open reading frame 2; Amphoterin-induced protein 2; DEGA; Differentially expressed in gastric adenocarcinoma; Differentially expressed in gastric adenocarcinomas; Transmembrane protein AMIGO 2; Transmembrane protein AMIGO2
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for depolarization-dependent survival of cultured cerebellar granule neurons. May mediate homophilic as well as heterophilic cell-cell interaction with AMIGO1 or AMIGO3. May contribute to signal transduction through its intracellular domain. May be required for tumorigenesis of a subset of gastric adenocarcinomas.
Gene References into Functions
  1. Amigo2, which is a member of the Amigo family that shows high species conservation, may be involved in determining liver metastasis by preferential adhesion of the tumour cells to liver endothelial cells. PMID: 28272394
  2. this study uncovers mechanisms underlying the therapeutic effects of BETi in melanoma and reveals the AMIGO2-PTK7 axis as a targetable pathway for metastatic melanoma. PMID: 29149598
  3. AMIGO2 is an important regulator of the PDK1-Akt pathway. PMID: 26553931
  4. Expression of ali1 promotes depolarization-dependent survival of cerebellar granule neurons. Mouse ali1 mapped to a chromosome 15 locus syntenic to candidate loci for familial Alzheimer's disease type 5 and Parkinson's disease 8 on human chromosome 12. PMID: 12843293
  5. Stable expression of a DEGA/AMIGO-2 antisense construct led to altered morphology, increased ploidy, chromosomal instability, decreased cell adhesion/migration, and a nearly complete abrogation of tumorigenicity in nude mice. PMID: 15107827

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Nucleus.
Protein Families
Immunoglobulin superfamily, AMIGO family
Tissue Specificity
Highest levels in breast, ovary, cervix, and uterus. Lower levels in lung, colon, and rectum. Differentially expressed in 56% of thyroid, 57% of pancreatic and 45% of stomach cancers.
Database Links

HGNC: 24073

OMIM: 615690

KEGG: hsa:347902

STRING: 9606.ENSP00000266581

UniGene: Hs.121520

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