Recombinant Human Amyloid-like protein 1 (APLP1), partial

Code CSB-YP001911HU1
MSDS
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Source Yeast
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Code CSB-EP001911HU1
MSDS
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Source E.coli
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Code CSB-EP001911HU1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP001911HU1
MSDS
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Source Baculovirus
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Code CSB-MP001911HU1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
APLP1
Uniprot No.
Alternative Names
APLP1; Amyloid beta precursor like protein 1; Amyloid beta; A4 precursor-like protein 1; Amyloid-like protein 1; APLP; APLP-1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in postsynaptic function. The C-terminal gamma-secretase processed fragment, ALID1, activates transcription activation through APBB1 (Fe65) binding. Couples to JIP signal transduction through C-terminal binding. May interact with cellular G-protein signaling pathways. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I.; The gamma-CTF peptide, C30, is a potent enhancer of neuronal apoptosis.
Gene References into Functions
  1. The direct cleavage of APLP1 is a novel feature of the gamma-secretase. PMID: 29382944
  2. Results provide direct evidence that APLP1 functions as a neuronal zinc-dependent adhesion protein and allow a more detailed understanding of the molecular mechanisms driving the formation of APLP1 adhesion platforms. PMID: 29021345
  3. The results show that in cognitively normal young adults carrying APlp1 mutations showed different spontaneous brain activity patterns without cerebral structural differences. PMID: 28987665
  4. These data reveal a conserved mechanism for the binding of APP-family proteins to HS and imply a specific regulatory role of HS modifications in the biology of APP and APP-like proteins. PMID: 25760599
  5. APLP1 and APLP2, behave similarly to APP in that they both associate with assembled NMDA receptors in the endoplasmic reticulum PMID: 25683482
  6. CSF levels of 3 endogenous peptides derived from APLP1 (APL1beta25, APL1beta27 and APL1beta28) were decreased in Down syndrome. PMID: 24740518
  7. APLP1 has a regulatory role in the nuclear translocation of APP family intracellular domains due to the sequestration of Fe65. PMID: 23874953
  8. [review] APP and its mammalian homologs, amyloid precursor-like proteins APLP1 and APLP2, participate under physiological conditions via trans-cellular dimerization in synaptogenesis. PMID: 21952790
  9. APLP1 binds the II-III loop of the Ca(v)2.3 calcium channel and that this binding promotes internalization of the channel. PMID: 22178872
  10. Both APLP1 and APLP2, form transcriptionally active triple protein complexes with Mint3 and transcriptional co-activators Taz andYap. PMID: 21178287
  11. The 2.1 A resolution electron density map reveals phosphate ions that are bound to the protein surface. Mutational analysis shows that protein residues interacting with the phosphate ions are also involved in heparin binding. PMID: 21574595
  12. Human cerebrospinal fluid contains three APLP1-derived Abeta-like peptides that are generated by beta- and gamma-cleavages at a concentration of approximately 4.5 nM. PMID: 20049724
  13. APLP1 and APLP2 are processed by the gamma-secretase in a Presenilin 1-dependent manner and the extreme carboxyl-terminal fragments produced by this processing (APP-like Intracellular Domain) are able to enhance Fe65-dependent gene activation PMID: 12228233
  14. APLP1 does not undergo the same type of regulated processing as APP and APLP2. PMID: 14597230
  15. APLP1 and APLP2 and APP are processed similarly to act via the same nuclear target and are regulated by BACE 1 in neurons PMID: 14699153
  16. APLP-1 and APLP-2 are processed by alpha- and gamma-secretase-like cleavages, and their intracellular domains can be released by cleavage at epsilon-sites. APLP-2 processing appears to be the most elaborate and to involve alternative cleavage sites. PMID: 14970212
  17. The 5' UTR of the Aplp1 gene lacks any type of CAGA box. This may explain its inability to form amyloid plaques. PMID: 15208260
  18. APLP1 affects the endocytosis of APP and makes more APP available for alpha-secretase cleavage PMID: 16344553
  19. APLP1 is differentially upregulated in gastrointestinal neuroendocrine tumours and may be important for the dissemination of small intestinal carcinoids PMID: 18430897
  20. Coexpression of APP with APLP1 or APLP2 leads to diminished generation of Abeta42 PMID: 19126676
  21. neither cell-derived nor chemically synthesized APLP1-derived peptide influences the oligomerization or aggregation of Abeta PMID: 19401174

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.; [C30]: Cytoplasm. Note=C-terminally processed in the Golgi complex.
Protein Families
APP family
Tissue Specificity
Expressed in the cerebral cortex where it is localized to the postsynaptic density (PSD).
Database Links

HGNC: 597

OMIM: 104775

KEGG: hsa:333

STRING: 9606.ENSP00000221891

UniGene: Hs.74565

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