Recombinant Human Ankyrin repeat domain-containing protein 54 (ANKRD54)

Code CSB-YP740682HU
MSDS
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Source Yeast
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Code CSB-EP740682HU
MSDS
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Source E.coli
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Code CSB-EP740682HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP740682HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP740682HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANKRD54
Uniprot No.
Alternative Names
ANKRD54; LIARAnkyrin repeat domain-containing protein 54; Lyn-interacting ankyrin repeat protein
Species
Homo sapiens (Human)
Expression Region
2-300
Target Protein Sequence
AAAAGDADD EPRSGHSSSE GECAVAPEPL TDAEGLFSFA DFGSALGGGG AGLSGRASGG AQSPLRYLHV LWQQDAEPRD ELRCKIPAGR LRRAARPHRR LGPTGKEVHA LKRLRDSANA NDVETVQQLL EDGADPCAAD DKGRTALHFA SCNGNDQIVQ LLLDHGADPN QRDGLGNTPL HLAACTNHVP VITTLLRGGA RVDALDRAGR TPLHLAKSKL NILQEGHAQC LEAVRLEVKQ IIHMLREYLE RLGQHEQRER LDDLCTRLQM TSTKEQVDEV TDLLASFTSL SLQMQSMEKR
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays an important role in regulating intracellular signaling events associated with erythroid terminal differentiation.
Gene References into Functions
  1. the results show that the interaction between BTK and ANKRD54 is highly selective, since it was also identified in a screen using human SH3-domainome. A novel finding is that BTK not only binds to ANKRD54, but stands out as the preferred interactor, being highly dominant over all other human SH3-domains. PMID: 28369144
  2. Liar is the first protein identified that specifically influences the nucleocytoplasmic shuttling of Btk and Txk and belongs to a rare group of known proteins carrying out this activity in a Crm1-dependent manner. PMID: 22527282
Subcellular Location
Nucleus. Cytoplasm. Midbody.
Database Links

HGNC: 25185

OMIM: 613383

KEGG: hsa:129138

STRING: 9606.ENSP00000215941

UniGene: Hs.135259

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