Code | CSB-EP001617HU |
Size | US$306 |
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The gene coding for the Human ALOX12 protein (1-663aa) is introduced into a plasmid vector, creating recombinant plasmid that is subsequently transformed into e.coli cells. e.coli cells capable of surviving in the presence of a specific antibiotic are selected and then cultured under conditions favorable for the expression of the gene of interest. A N-terminal 6xHis tag is attached to the protein. Post-expression, the recombinant Human ALOX12 protein is isolated and purified from the cell lysate using affinity purification. Denaturing SDS-PAGE is applied to resolve the resulting recombinant Human ALOX12 protein, revealing a purity exceeding 90%.
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We ordered your following product CSB-EP001617HU and have the following questions:
1.Need to lyophylized and reconstitute in Tris Buffer. Could you help me with the best protocol for this enzyme?
2. For how long is this recombinant 12-LOX stable in Tris Buffer?