Recombinant Human Arsenite methyltransferase (AS3MT)

1. combined effect of N6AMT1 haplotype 2_GGCCAT and As3MT haplotype 2_GCAC showed consistence with the additive significance of each haplotype on % iAs: the mean was 5.47% and 9.36% for carriers with both and null haplotypes, respectively PMID: 27637898
2. Maternal alleles for five of the seven SNPs (rs7085104, rs3740400, rs3740393, rs3740390, and rs1046778) were associated with urinary concentrations of iAs metabolites, and alleles for one SNP (rs3740393) were associated with birth outcomes/measures. These associations were strongly dependent upon the male sex of the fetus but independent of fetal genotype for AS3MT. PMID: 26928318
3. The combined analysis confirmed evidence of genome-wide significant associations in the Han Chinese population for three loci, at 2p16.1 (rs1051061, in an exon of VRK2, P=1.14 x 10-12, odds ratio (OR)=1.17), 6p22.1 (rs115070292 in an intron of GABBR1, P=4.96 x 10-10, OR=0.77) and 10q24.32 (rs10883795 in an intron of AS3MT, P=7.94 x 10-10, OR=0.87; rs10883765 at an intron of ARL3, P=3.06 x 10-9, OR=0.87). PMID: 27922604
4. AS3MT and N6AMT1 polymorphisms and urinary arsenic metabolites (%iAs, %MMA, %DMA) in 722 subjects from an arsenic-cancer case-control study in a uniquely exposed area in northern Chile, were examined. PMID: 28640505
5. Risk alleles spanning multiple genes across the 10q24.32 schizophrenia-related locus are associated in the human brain selectively with an increase in the expression of both BLOC-1 related complex subunit 7 (BORCS7) and a previously uncharacterized, human-specific arsenite methyltransferase (AS3MT) isoform (AS3MTd2d3). PMID: 27158905
6. A joint dose-response effect was identified for a AS3MT high-risk haplotype and inefficient arsenic methylation capacity on developmental delay. PMID: 28235556
7. genome-wide association study in population in United States (AZ, OK, SD, ND): Data suggest SNPs in AS3MT (rs17878846, rs10509760) partly explain inter-individual variability in susceptibility to arsenic poisoning (and urine arsenic species patterns). PMID: 26209557
8. Its genotype-dependent differences in arsenic metabolism is noted.(review) PMID: 26411936
9. The present study found that rs3740400 in As3MT and rs156697 in GSTO2 were strongly associated with the arsenic species (%iAs, %MMA and MMA/iAs and DMA/MMA ratios) in urine of the occupationally exposed workers and they may influence methylation capacity. PMID: 25491248
10. analysis of the functional roles of GSH and the crucial Cys residues in iAs3+ methylation catalyzed by hAS3MT PMID: 25349987
11. a strong signal of positive selection in the main arsenic methyltransferase AS3MT gene, which has been previously associated with lower concentrations of the most toxic product of arsenic metabolism monomethylarsonic acid. PMID: 26366667
12. This study's results provide preliminary evidence for the over-transmission of the A allele at the AS3MT rs11191454 polymorphism in Attention Deficit Hyperactivity Disorder. PMID: 25461954
13. Influence of the level of arsenic exposure and the presence of T860C polymorphism in human As urinary metabolic profile PMID: 24845704
14. Functions of arsenic (III) methyltransferase S-adenosylmethionine binding site were evaluated. PMID: 25447140
15. Genetic variation in AS3MT gene is associated with arsenic metabolism and risk of basal cell carcinoma. PMID: 25156000
16. Two SNPs, AS3MT 12390 (rs3740393) and 14458 (rs11191439) were consistently related to arsenic methylation regardless of the populations examined for the analysis. PMID: 21731446
17. AS3MT SNPs were associated with arsenic metabolism efficiency and arsenic toxicity in exposed individuals. PMID: 24536095
18. Low-level arsenic exposure is associated with cognitive functioning; however, this association is modified by an AS3MT gene. PMID: 24621105
19. A hypomethylated region in the AS3MT promoter was associated with higher arsenic exposure.Arsenic exposure influences the epigenetic regulation of AS3MT. PMID: 24154821
20. polymorphisms may impact disease susceptibility [review] PMID: 24792412
21. when workers were classified according to their AS3MT Met287Thr genotypes, significantly higher micronucleus values were for those carrying the variant allele [odds ratio (OR), 3.4 (1.6-5.2); P=0.0003)] PMID: 24361376
22. Genetic polymorphisms in AS3MT gene in a Western Chinese population influenced urinary arsenic metabolism from arsenic in drinking water. PMID: 24239724
23. Five N6AMT1 single nucleotide polymorphisms and two N6AMT1 haplotypes were significantly associated with the percentage of methylarsonic acid in urine, even after adjusting for arsenic (+3 oxidation state) methyltransferase haplotype. PMID: 23665909
24. Residues Asp76, 84, 102 and 150 greatly influence hAS3MT catalytic activity, affecting S-adenosylmethionine-binding or methyl transfer. PMID: 23742935
25. Individuals with M287T and G4965C AS3MT polymorphisms had higher levels of urinary DMAs(III) and were more frequently diabetic than the respective wild-type carriers, although the excess was not statistically significant PMID: 23093101
26. Data raise the possibility that, during a few thousand years, natural selection for tolerance to the environmental stressor arsenic may have increased the frequency of protective variants of AS3MT. PMID: 23070617
27. the AS3MT haplotype status strongly predicted DNA methylation and gene expression of AS3MT as well as several genes in 10q2 PMID: 23341986
28. Single nucleotide polymorphisms (SNPs) in arsenic methyltransferase and methylene-tetrahydrofolate reductase is associated with bladder cancer in those exposed to low concentrations of inorganic arsenic. SNPs in glutathione S-transferase omega-1 are not. PMID: 22747749
29. analysis of rapid equilibrium kinetics of arsenite methylation catalyzed by recombinant human arsenic (+3 oxidation state) methyltransferase (hAS3MT) PMID: 22955273
30. structural insights into the mechanism of substrate binding and catalysis PMID: 22712827
31. Pregnancy and the AS3MT genotype independently influence the arsenic methylation phenotype PMID: 22547080
32. Genetic polymorphisms GSTO and As3MT modify arsenic metabolism as evidenced by altered urinary arsenic excretion PMID: 22339537
33. Coronary heart disease and its main risk factors were associated with low-level arsenic exposure, AS3MT polymorphism or both. Hypertension was associated with higher arsenic exposure, while hyperlipidemia was associated AS3MT polymorphism. PMID: 22341486
34. These data suggest that the expression pattern of splicing variants of the As3MT gene may affect the capacity for arsenic methylation in cells. PMID: 22005461
35. investigation of the roles of AS3MT in the effects of arsenicals, carcinogens found ubiquitously in nature: metabolism, toxicity, and accumulation of arsenicals in hepatocytes PMID: 21537954
36. Polymorphisms in AS3MT significantly predicted As metabolism across these two very different populations, suggesting that AS3MT may have an impact on As metabolite patterns in populations worldwide. PMID: 21247820
37. Distribution of 18 SNPs in AS3MT was assessed in healthy individuals in Mexico and Germany: 38 Mestizo, 69 Nahuas, 50 Huicholes, and 32 Germans. PMID: 20571777
38. enzymatic activity, kinetics, thermal stability and secondary structures of mutant enzyme PMID: 20971157
39. an ordered sequence for the binding of SAM and arsenite to the hAS3MT; whereas, no interactions between GSH and the hAS3MT were detected. PMID: 20621156
40. As3MT effects organic arsenic metabolism PMID: 20532380
41. Investigation of 46 SNPs [Mexico]: Genetic association analysis with arsenic metabolism confirmed the previously observed association between AS3MT variants, including this large cluster of linked polymorphisms, and arsenic methylation efficiency PMID: 20014157
42. Data suggest that the inhibitory effect of the ions (Co(2+), Mn(2+), and Zn(2+)) or selenite on AS3MT activity might be via the interactions of them with free Cys residues in human AS3MT to form inactive protein adducts. PMID: 20129672
43. The present study is the first to demonstrate the existence of genetic heterogeneity in a world wide distribution of 18 single nucleotide polymorphisms in AS3MT PMID: 19932709
44. Heterologous expression of rat AS3MT in human urothelial cells...confers capacity to methylate inorganic arsenic and alters its cytotoxicity and that of methylated arsenicals. PMID: 15808521
45. a strong genetic association between polymorphisms of CYT19 and D:M ratio (urinary dimethylarsenic(V) to monomethylarsenic(V))in drinking water exposed Mexican children, not adults. PMID: 15929903
46. inherited variation in AS3MT may contribute to variation in arsenic metabolism PMID: 16407288
47. is strongly associated with the ratio of urinary dimethylarsinic acid to monomethylarsonic acid (D/M) in children (7-11 years) but not in adults (18-79 years) PMID: 17306849
48. Our results indicate that genetic polymorphisms in AS3MT contribute to inter-individual variation in arsenic biotransformation and, therefore, may contribute to inter-individual variations in risk of arsenic toxicity and arsenic carcinogenesis. PMID: 17850829
49. mutation frequencies of AS3MT in Asian populations were relatively lower than those of African and Caucasian populations: frequencies of mutation in the Mongolian, Korean, and Japanese populations were 0.040, 0.010, and 0.010, respectively PMID: 17889916
50. Individuals with the AS3MT(287)Thr variant display increased arsenic methylation and thus associated with increased risk for the toxic and genotoxic effects of arsenic exposure PMID: 18334919

Show More

Hide All

HGNC: 17452

OMIM: 611806

KEGG: hsa:57412

STRING: 9606.ENSP00000358896

UniGene: Hs.720370