Recombinant Human Basal cell adhesion molecule (BCAM), partial

Code CSB-YP341920HU
MSDS
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Source Yeast
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Code CSB-EP341920HU
MSDS
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Source E.coli
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Code CSB-EP341920HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341920HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP341920HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BCAM
Uniprot No.
Alternative Names
Antigen identified by monoclonal F8 ; AU; Auberger B antigen; B CAM cell surface glycoprotein; B cell adhesion molecule; B-CAM cell surface glycoprotein; Basal cell adhesion molecule (Lu and Au blood groups); Basal cell adhesion molecule (Lutheran blood group); Basal cell adhesion molecule; Basal cell adhesion molecule Lu and Au blood groups; Basal cell adhesion molecule Lutheran blood group; Bcam; BCAM_HUMAN; CD239; CD239 antigen; F8/G253 antigen; Glycoprotein 95kDa; LU; Lutheran; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group Auberger b antigen included ; Lutheran blood group glycoprotein; MSK19
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Laminin alpha-5 receptor. May mediate intracellular signaling.
Gene References into Functions
  1. Lu expression by erythrocytes from patients with myeloproliferative neoplasms and splanchnic venous thrombosi is significantly increased compared to erythrocytes from healthy individuals PMID: 29756283
  2. This is the first report showing that Lu/BCAM, in the presence of its ligand laminin, is oncogenic in human urothelial cancers and may have potential as a novel therapeutic target. PMID: 28841878
  3. The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
  4. four new alleles with coding sequence variants were identified in the LU gene PMID: 27043150
  5. Cytometry analysis evidenced a specific expression profile on reticulocytes of SCA infants, with notably an increased expression of the adhesion molecules Lu/BCAM, ICAM-4 and LFA-3, both in percentage of positive cells and in surface density. PMID: 26137540
  6. These studies revealed amino acids 720 to 1014 of CNF1 as the binding site for Lu/BCAM. PMID: 24453976
  7. High BCAM expression is associated with hepatocellular carcinoma. PMID: 25051049
  8. Adhesion of sickle reticulocytes to HC-treated endothelial cells was decreased despite the HC-derived increase of Lu/BCAM expression. PMID: 24616094
  9. tumor cell migration on LM-511 requires that Lu/B-CAM competitively modulates cell attachment through integrins. PMID: 24036115
  10. DARC and BCAM expression was associated with pathogenesis of thyroid carcinoma and correlated with clinical-pathological features. PMID: 23168236
  11. Glu425 in the Lu glycoprotein is required for expression of Lu7, and Ala425 is associated with the LU:-7 phenotype. This completes the molecular basis associated with all antigens known to be in the Lutheran blood group system. PMID: 23421542
  12. Increases in adhesion frequency provide significant evidence of the role of epinephrine in BCAM/Lu-laminin and ICAM-4-alpha(v)beta(3) bonding, and suggest mechanisms of vaso-occlusion during physical exertion in sickle cell trait. PMID: 22404936
  13. Aggregation of mononuclear and red blood cells through an {alpha}4{beta}1-Lu/basal cell adhesion molecule interaction in sickle cell disease PMID: 20562314
  14. BCAM glycoproteins are the unique erythroid receptors of laminin alpha5 chain, a major component of the extracellular matrix and are involved in several erythrocyte diseases. PMID: 20655789
  15. Results demonstrate that the alpha5 LG3 module is essential for the Lutheran blood group glycoprotein (Lu) binding to laminin alpha5. PMID: 12244066
  16. B-CAM/LU is the most critical receptor mediating adhesion to laminin under both static and flow conditions in sickle cell anemia. PMID: 14755370
  17. interactions of B-CAM and laminin may be involved in progression of epithelial skin tumors PMID: 15278364
  18. Lu mediates the adhesion of human endothelial cells to alpha5 laminins in collaboration with integrins beta(1) and alpha(v)beta(3). PMID: 16236823
  19. B-CAM spliceoform seems to be overexpressed by a variety of different malignant tumors and may be involved, along with other adhesion receptor proteins, in malignant transformation and tumor metastasis. (review) PMID: 16584446
  20. The Laminin 511/521-binding site on the Lutheran blood group glycoprotein is located at the flexible junction of Ig domains 2 and 3. PMID: 17638854
  21. study presents a combined modeling & small angle X-ray scattering approach that extends the structural analysis of Lu gp to all five extracellular domains & provides further insight to its interaction with laminins (Ln511 & Ln521) PMID: 18302987
  22. Lu/BCAM gps expressed on erythroid or on endothelial cells are involved in SS RBC-endothelium interactions & could play a role in abnormal adhesion of SS RBCs to vascular endothelium contributing to vaso-occlusive crises reported for sickle cell disease. PMID: 18514010
  23. the interaction of the Lu cytoplasmic tail with the cytoskeleton regulates its adhesive receptor function PMID: 18815288
  24. CD239 was expressed in a constitutive fashion in polycythaemia vera-derived bone marrow cells and thus a pro-thrombotic function does not seem to be mediated by increased CD239 expression alone. PMID: 18972067
  25. Lutheran blood group glycoprotein (Lu) is one of the proteins cleaved by MT1-MMP. PMID: 19667067

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Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.
Database Links

HGNC: 6722

OMIM: 111200

KEGG: hsa:4059

STRING: 9606.ENSP00000270233

UniGene: Hs.625725

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