Product Details

Uniprot No.

Target Names

Alternative Names

Antigen identified by monoclonal antibody F8 antibody; AU antibody; Auberger B antigen antibody; B CAM cell surface glycoprotein antibody; B cell adhesion molecule antibody; B-CAM cell surface glycoprotein antibody; Basal cell adhesion molecule (Lu and Au blood groups) antibody; Basal cell adhesion molecule (Lutheran blood group) antibody; Basal cell adhesion molecule antibody; Basal cell adhesion molecule Lu and Au blood groups antibody; Basal cell adhesion molecule Lutheran blood group antibody; Bcam antibody; BCAM_HUMAN antibody; CD239 antibody; CD239 antigen antibody; F8/G253 antigen antibody; Glycoprotein 95kDa antibody; LU antibody; Lutheran antibody; Lutheran antigen antibody; Lutheran blood group (Auberger b antigen included) antibody; Lutheran blood group Auberger b antigen included antibody; Lutheran blood group glycoprotein antibody; MSK19 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human BCAM protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

1G12

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

BCAM, also known as the basal cell adhesion molecule or Lutheran blood group glycoprotein, serves as a critical laminin receptor that mediates cell-matrix interactions in various tissues. This immunoglobulin superfamily member plays significant roles in vascular biology, where it facilitates adhesion of red blood cells to endothelial laminin, and has garnered considerable research interest for its involvement in sickle cell disease pathophysiology and tumor cell migration. Understanding BCAM expression patterns and function provides valuable insights into both normal physiological processes and disease mechanisms affecting the cardiovascular system.

This recombinant monoclonal antibody, clone 1G12, offers researchers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and produced from a stable expression system, you can expect consistent performance across experiments and over time, eliminating the lot-to-lot variability that can complicate long-term studies or multi-site collaborations. The antibody is raised in rabbit against a synthetic peptide derived from human BCAM and has been affinity-purified to ensure high specificity.

Validation studies demonstrate reliable performance in immunohistochemistry applications on formalin-fixed, paraffin-embedded human tissues. Testing in human kidney tissue and human prostate cancer specimens at 1:100 dilution using citrate buffer antigen retrieval confirms the antibody's utility for examining BCAM expression in both normal and pathological contexts. The recommended working dilution range of 1:50 to 1:200 provides flexibility for optimization across different tissue types and detection systems.

For cardiovascular researchers investigating laminin-mediated adhesion, blood group antigen biology, or tumor progression studies where BCAM expression may influence metastatic potential, this antibody provides a dependable tool for immunohistochemical analysis of human specimens.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Laminin alpha-5 receptor. May mediate intracellular signaling.

Gene References into Functions

Lu expression by erythrocytes from patients with myeloproliferative neoplasms and splanchnic venous thrombosi is significantly increased compared to erythrocytes from healthy individuals PMID: 29756283
This is the first report showing that Lu/BCAM, in the presence of its ligand laminin, is oncogenic in human urothelial cancers and may have potential as a novel therapeutic target. PMID: 28841878
The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower. PMID: 26898119
four new alleles with coding sequence variants were identified in the LU gene PMID: 27043150
Cytometry analysis evidenced a specific expression profile on reticulocytes of SCA infants, with notably an increased expression of the adhesion molecules Lu/BCAM, ICAM-4 and LFA-3, both in percentage of positive cells and in surface density. PMID: 26137540
These studies revealed amino acids 720 to 1014 of CNF1 as the binding site for Lu/BCAM. PMID: 24453976
High BCAM expression is associated with hepatocellular carcinoma. PMID: 25051049
Adhesion of sickle reticulocytes to HC-treated endothelial cells was decreased despite the HC-derived increase of Lu/BCAM expression. PMID: 24616094
tumor cell migration on LM-511 requires that Lu/B-CAM competitively modulates cell attachment through integrins. PMID: 24036115
DARC and BCAM expression was associated with pathogenesis of thyroid carcinoma and correlated with clinical-pathological features. PMID: 23168236
Glu425 in the Lu glycoprotein is required for expression of Lu7, and Ala425 is associated with the LU:-7 phenotype. This completes the molecular basis associated with all antigens known to be in the Lutheran blood group system. PMID: 23421542
Increases in adhesion frequency provide significant evidence of the role of epinephrine in BCAM/Lu-laminin and ICAM-4-alpha(v)beta(3) bonding, and suggest mechanisms of vaso-occlusion during physical exertion in sickle cell trait. PMID: 22404936
Aggregation of mononuclear and red blood cells through an {alpha}4{beta}1-Lu/basal cell adhesion molecule interaction in sickle cell disease PMID: 20562314
BCAM glycoproteins are the unique erythroid receptors of laminin alpha5 chain, a major component of the extracellular matrix and are involved in several erythrocyte diseases. PMID: 20655789
Results demonstrate that the alpha5 LG3 module is essential for the Lutheran blood group glycoprotein (Lu) binding to laminin alpha5. PMID: 12244066
B-CAM/LU is the most critical receptor mediating adhesion to laminin under both static and flow conditions in sickle cell anemia. PMID: 14755370
interactions of B-CAM and laminin may be involved in progression of epithelial skin tumors PMID: 15278364
Lu mediates the adhesion of human endothelial cells to alpha5 laminins in collaboration with integrins beta(1) and alpha(v)beta(3). PMID: 16236823
B-CAM spliceoform seems to be overexpressed by a variety of different malignant tumors and may be involved, along with other adhesion receptor proteins, in malignant transformation and tumor metastasis. (review) PMID: 16584446
The Laminin 511/521-binding site on the Lutheran blood group glycoprotein is located at the flexible junction of Ig domains 2 and 3. PMID: 17638854
study presents a combined modeling & small angle X-ray scattering approach that extends the structural analysis of Lu gp to all five extracellular domains & provides further insight to its interaction with laminins (Ln511 & Ln521) PMID: 18302987
Lu/BCAM gps expressed on erythroid or on endothelial cells are involved in SS RBC-endothelium interactions & could play a role in abnormal adhesion of SS RBCs to vascular endothelium contributing to vaso-occlusive crises reported for sickle cell disease. PMID: 18514010
the interaction of the Lu cytoplasmic tail with the cytoskeleton regulates its adhesive receptor function PMID: 18815288
CD239 was expressed in a constitutive fashion in polycythaemia vera-derived bone marrow cells and thus a pro-thrombotic function does not seem to be mediated by increased CD239 expression alone. PMID: 18972067
Lutheran blood group glycoprotein (Lu) is one of the proteins cleaved by MT1-MMP. PMID: 19667067

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Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Wide tissue distribution (highest in the pancreas and very low in brain). Closely associated with the basal layer of cells in epithelia and the endothelium of blood vessel walls.

Database Links

HGNC: 6722

OMIM: 111200

KEGG: hsa:4059

STRING: 9606.ENSP00000270233

UniGene: Hs.625725

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Code	CSB-RA958435A0HU
Size	US$210
	Order now
Image	IHC image of CSB-RA958435A0HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA958435A0HU diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.
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BCAM Recombinant Monoclonal Antibody

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Target Background