Recombinant Human Beta-1,4-galactosyltransferase 3 (B4GALT3), partial

Code CSB-RP007044h
MSDS
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
B4GALT3
Uniprot No.
Research Area
Metabolism
Alternative Names
4-galactosyltransferase 3; 4-galactosyltransferase; 4-GalTase 3; b4Gal T3; b4Gal-T3; B4GALT3; B4GT3_HUMAN; Beta 1,4 galactosyltransferase 3 ; Beta 1,4 GalTase 3; Beta-1; Beta-N-acetylglucosaminyl-glycolipid beta-1; Beta4Gal T3; Beta4Gal-T3; Nal synthase; UDP Gal:beta GlcNAc beta-1,4 galactosyltransferase 3; UDP-Gal:beta-GlcNAc beta-1; UDP-galactose:beta-N-acetylglucosamine beta-1
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
34-393aa
Target Protein Sequence
LSALFGRDQGPTFDYSHPRDVYSNLSHLPGAPGGPPAPQGLPYCPERSPLLVGPVSVSFSPVPSLAEIVERNPRVEPGGRYRPAGCEPRSRTAIIVPHRAREHHLRLLLYHLHPFLQRQQLAYGIYVIHQAGNGTFNRAKLLNVGVREALRDEEWDCLFLHDVDLLPENDHNLYVCDPRGPRHVAVAMNKFGYSLPYPQYFGGVSALTPDQYLKMNGFPNEYWGWGGEDDDIATRVRLAGMKISRPPTSVGHYKMVKHRGDKGNEENPHRFDLLVRTQNSWTQDGMNSLTYQLLARELGPLYTNITADIGTDPRGPRAPSGPRYPPGSSQAFRQEMLQRRPPARPGPLSTANHTALRGSH
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
67.2kDa
Protein Length
Partial
Tag Info
N-terminal GST-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids.
Gene References into Functions
  1. High-metastatic hepatocellular carcinoma cells secrete exosomal miR-1247-3p that directly targets B4GALT3, leading to activation of beta1-integrin-NF-kappaappaB metastatic signaling in fibroblasts resulting in lung cancer. PMID: 29335551
  2. The mutations c.1456C < T (p.L486F) in MYOC and c.322G < A (p.V108I) in B4GALT3 are likely responsible for the pathogenesis of Primary Open-angle Glaucoma in this family. PMID: 27900994
  3. our findings evidenced that B4GALT3 upregulated by miR-27a contributes to the tumorigenic activities by b1-integrin pathway and might provide potential biomarkers for cervical cancer. PMID: 26987623
  4. Data suggest that expression of B4GALT3 in placenta is up-regulated in third trimester; over-expression of B4GALT3 in cultured trophoblasts suppresses cell migration; thus, B4GALT3 appears to regulate trophoblast invasion in late stages of pregnancy. PMID: 25659296
  5. beta-1,4-Galactosyltransferase III suppresses beta1 integrin-mediated invasive phenotypes and negatively correlates with metastasis in colorectal cancer. PMID: 24403309
  6. beta-1,4-Galactosyltransferase III enhances invasive phenotypes via beta1-integrin and predicts poor prognosis in neuroblastoma. PMID: 23444218

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Subcellular Location
Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Trans cisternae of Golgi stack.
Protein Families
Glycosyltransferase 7 family
Tissue Specificity
Found in various tissues. Highest expression in placenta, prostate, testis, ovary, intestine and muscle, and in fetal brain.
Database Links

HGNC: 926

OMIM: 604014

KEGG: hsa:8703

STRING: 9606.ENSP00000320965

UniGene: Hs.321231

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