Recombinant Human Bromodomain adjacent to zinc finger domain protein 1A (BAZ1A), partial

Code CSB-YP885707HU
MSDS
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Source Yeast
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Code CSB-EP885707HU
MSDS
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Source E.coli
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Code CSB-EP885707HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885707HU
MSDS
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Source Baculovirus
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Code CSB-MP885707HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BAZ1A
Uniprot No.
Alternative Names
Acf1; ACF1; drosophila; homolog of; ATP dependent chromatin remodelling protein; ATP utilizing chromatin assembly and remodeling factor 1; ATP-dependent chromatin-remodeling protein; ATP-utilizing chromatin assembly and remodeling factor 1; B930060C03; Baz1a; BAZ1A_HUMAN; BC065123; Bromodomain adjacent to zinc finger domain 1A; Bromodomain adjacent to zinc finger domain protein 1A; cbp146; CHRAC subunit ACF1; DKFZp586E0518; FLJ14383; Gtl5; hACF1; hWALp1; WALp1; WCRF180; Williams syndrome transcription factor related chromatin remodeling factor 180; Williams syndrome transcription factor-related chromatin-remodeling factor 180
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory subunit of the ATP-dependent ACF-1 and ACF-5 ISWI chromatin remodeling complexes, which form ordered nucleosome arrays on chromatin and slide edge- and center-positioned histone octamers away from their original location on the DNA template to facilitate access to DNA during DNA-templated processes such as DNA replication, transcription, and repair. Both complexes regulate the spacing of nucleosomes along the chromatin and have the ability to slide mononucleosomes to the center of a DNA template in an ATP-dependent manner. The ACF-1 ISWI chromatin remodeling complex has a lower ATP hydrolysis rate than the ACF-5 ISWI chromatin remodeling complex. Has a role in sensing the length of DNA which flank nucleosomes, which modulates the nucleosome spacing activity of the ACF-5 ISWI chromatin remodeling complex. Involved in DNA replication and together with SMARCA5/SNF2H is required for replication of pericentric heterochromatin in S-phase. May have a role in nuclear receptor-mediated transcription repression.
Gene References into Functions
  1. Results from the study on gene variability in early stage embryos identifies BAZ1A as a putative variability marker of the 8-cell embryo stage. PMID: 26288249
  2. Results from the study suggest that acute cocaine exposure increased Baz1a expression in nucleus accumbens while repeated cocaine exposure produced the opposite effect. Findings implicate BAZ1A in molecular and behavioral plasticity to cocaine and offer new insight into the pathophysiology of cocaine addiction. PMID: 28412501
  3. promotes recovery after DNA damage, in part by recruiting SMARCA5 to damaged chromatin PMID: 29021563
  4. Study reports de novo mutation in BAZ1A, in a patient with a syndromic form of intellectual disability (ID). This mutation affects the expression of genes involved in several biological pathways such as vitamin D regulation, Wnt signaling, and postsynaptic signaling. Data point to an important role for BAZ1A in neurodevelopment, and highlight a possible link for BAZ1A to ID. PMID: 27328812
  5. CHD3.1 and ACF1-SNF2H display counteractive activities but similar histone affinities. PMID: 25533843
  6. ISWI proteins Snf2H, Snf2L as well as Acf1 accumulate at UV-induced DNA damage sites within tens of seconds and reach a plateau after a few minutes. PMID: 21738833
  7. hACF1-containing factors are more generally involved in the DNA damage response. PMID: 21745822
  8. The ACF1 and its complexes play important roles in DNA double-strand breaks (DSBs) repair. PMID: 21172662
  9. The data support a model in which the human ACF anf yeast RSC remodeling enzymes move nucleosomes to new locations by a general sequence-independent mechanism. PMID: 19450608
  10. ACF1 alters the remodeling strategy of SNF2h PMID: 16877760
  11. Has a critical role in the transcriptional repression of specific nuclear hormone receptor-regulated genes. PMID: 17519354

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Subcellular Location
Nucleus. Note=May target the CHRAC complex to heterochromatin.
Protein Families
WAL family
Tissue Specificity
Highly expressed in testis and at low or undetectable levels in other tissues analyzed.
Database Links

HGNC: 960

OMIM: 605680

KEGG: hsa:11177

STRING: 9606.ENSP00000353458

UniGene: Hs.509140

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