Recombinant Human Calcium-activated potassium channel subunit alpha-1(KCNMA1),partial

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Code CSB-EP614255HU
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  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP614255HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KCNMA1.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP614255HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) KCNMA1.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names KCNMA1
Uniprot No. Q12791
Research Area Cancer
Alternative Names subfamily M subunit alpha-1; BK channel; BKCA alpha; BKCA alpha subunit; BKTM; Calcium-activated potassium channel; Calcium-activated potassium channel subunit alpha-1; Drosophila slowpoke like; hSlo; K(VCA)alpha; KCa1.1; KCMA1_HUMAN; KCNMA; KCNMA1; Maxi K channel; Maxi Potassium channel alpha ; MaxiK; SAKCA; SLO alpha; SLO; Slo homolog; Slo-alpha; Slo1; Slowpoke homolog
Species Homo sapiens (Human)
Source E.coli
Expression Region 411-560aa
Target Protein Sequence VVCGHITLESVSNFLKDFLHKDRDDVNVEIVFLHNISPNLELEALFKRHFTQVEFYQGSVLNPHDLARVKIESADACLILANKYCADPDAEDASNIMRVISIKNYHPKIRIITQMLQYHNKAHLLNIPSWNWKEGDDAICLAELKLGFIA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 20.0 kDa
Protein Length Partial
Tag Info C-terminal 6xHis-HPC4-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Tris-based buffer,50% glycerol
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).
Gene References into Functions
  1. Data suggest that BKCa channels play important role in cell cycle progression and cell self-renewal in cardiac stem cells. PMID: 29211342
  2. KCNMA1 mutation causes epilepsy and changes voltage gating but not calcium sensitivity. PMID: 29330545
  3. Data suggest that large-conductance calcium-activated potassium channels, alpha subunit (BK channel) mutations affect the Ca2 +-sensing more than the voltage- sensing mechanism. PMID: 28966112
  4. An important role of BKCa in proliferation and migration of endometrial cancer HEC-1-B cells. PMID: 29477869
  5. Study identified a novel tumor suppressive gene, KCNMA1, which is frequently inactivated in gastric cancer because of promoter methylation. KCNMA1 exerts a tumor suppressive function by regulating the PTK2 expression to activate the PI3K-AKT pathway. PMID: 28231797
  6. High BKCA expression is associated with breast cancer. PMID: 26993604
  7. Heme-independent activation of the CFTR channel and the Slo1 BKCa channel by carbon monoxide (CO) may represent two different modes of gating regulation of ion channels by CO. In the case of CFTR, Fe3+ is bound to the R-ICL3 interface.15,16 This interfacial Fe3+ site is formed by traditional coordination ligands such as H950 and H954 from ICL3 and C832 and D836 from the R domain.[Review] PMID: 28474046
  8. High BK channel is associated with glioblastoma. PMID: 26893360
  9. Oxidant signaling underlies PKGIalpha modulation of Ca2+ sparks and BKCa in myogenically active arterioles PMID: 27729549
  10. These results provide further insights into the mechanism of modulation of the different N-terminal regions of the BKCa channel by beta-subunits. PMID: 28750098
  11. Thiourea derivative, compound 326, stimulates the activity of large-conductance, calcium-activated potassium channels. PMID: 28075010
  12. Implicating both KCa1.1 and KCa3.1 channels. PMID: 28028617
  13. These results identify KCa1.1 channels as crucial regulators of skeletal myogenesis. PMID: 27763639
  14. Results show that reduced miR-17 expression is correlated with increased expression of KCNMA1 in malignant pleural mesothelioma. Computational analysis identified KCNMA1 gene is a direct target of miR-17. PMID: 27245839
  15. These findings provide significant new evidence that BKalphaDeltae2 can modulate cellular responses to physiological stimuli in human chondrocyte and contribute under pathophysiological conditions, such as osteoarthritis. PMID: 27758860
  16. Our report defines a novel autosomal recessive KCNMA1-related epileptic phenotype that encompasses cerebellar atrophy without paroxysmal dyskinesia, and highlights the sensitivity of the developing brain to both increased and decreased activity of the KCNMA1-encoded channels PMID: 27567911
  17. A directed proteomic approach discovered the novel interaction of BKCa with Tom22, a component of the mitochondrion outer membrane import system, and the adenine nucleotide translocator (ANT). PMID: 27592226
  18. GSK3b may up-regulate BK channels, an effect disrupted by lithium or additional expression of AKT and possibly participating in the regulation of cell volume and excitability. PMID: 27537208
  19. MiR-31 represses KCNMA1 expression. PMID: 26386726
  20. By introducing lanthanide binding tags in the extracellular region of the alpha- or beta1-subunit, we determined (i) a basic extracellular map of the BK channel, (ii) beta1-subunit-induced rearrangements of the voltage sensor in alpha-subunits, and (iii) the relative position of the beta1-subunit within the alpha/beta1-subunit complex. PMID: 27217576
  21. Suggest that BK channel modulation by auxiliary gamma subunits depends on intra- and/or juxta-membrane mechanisms. PMID: 26009545
  22. Suberoylanilide hydroxamic acid enhanced the expression of malignant genes such as KCNMA1 in lung cancer cells remaining after treatment, creating a more drug-resistant state. PMID: 25796627
  23. We conclude that arachidonic acid itself selectively activates the beta1-associated BKCa channel, destabilizing its closed state probably by interacting with the beta1-subunit, without modifying the channel voltage sensitivity PMID: 24375290
  24. The present study revealed that 11,12-EET targets the TRPV4-TRPC1-KCa1.1 complex to induce smooth muscle cell hyperpolarization and vascular relaxation in human LIMAs. PMID: 25511389
  25. DS-201 selectively targets the pore-forming alpha subunit of human BKCa channels, thus enhancing the channel activities and increasing the subunit expression and trafficking. PMID: 25345746
  26. Bisphenol A activates BK alpha via an extracellular site and that Bisphenol A-sensitivity is increased by the beta1 subun PMID: 24476761
  27. show that Slo1 is localized to the sperm flagellum and is inhibited by progesterone. Inhibition of hKSper by progesterone may depolarize the spermatozoon to open the calcium channel CatSper PMID: 24137539
  28. our results strongly suggest that AT1R regulates BK channels through a close protein-protein interaction involving multiple BK regions and independent of G-protein activation. PMID: 25070892
  29. hSlo1c associates with Cav-1 in human brain microvascular endothelial cells.A 57-amino acid (966-1022) fragment in hSlo1c was identified to be critical for hSlo1c/Cav-1 interaction. PMID: 24705869
  30. Recombinant martentoxin selectively blocks KCNMA1/KCNMB4 channels. PMID: 24759175
  31. HIV and methamphetamine -induced elevated miR-9 lead to suppression of miR-response element-containing splice variants of KCNMA1 PMID: 23508624
  32. Overexpression of JAK2 in alveolar rhabdomyosarcoma cells significantly enhanced BK channel mRNA and protein abundance. PMID: 24696148
  33. The reduced KCa1.1 channel protein expression was also observed. PMID: 24260325
  34. Here we examined the action of docosahexaenoic acid on the Slo1 channel without any auxiliary subunit and sought to elucidate the biophysical mechanism and the molecular determinants of the DHA sensitivity. PMID: 24127525
  35. N-terminal isoforms of the large-conductance Ca(2)-activated K channel are differentially modulated by the auxiliary beta1-subunit. PMID: 24569989
  36. Overexpression of KCNMA1, the pore-forming subunit of BK, overcame the reduction of airway surface liquid volume induced by IFN-gamma. PMID: 24414257
  37. The SLO1 gating ring is not required for voltage activation but is required for Ca2+ and Mg2+ activation. PMID: 24067659
  38. A novel Cav3-KCa1.1 signaling complex has been identified where Cav3-mediated calcium entry enables KCa1.1 activation over a wide range of membrane potentials. PMID: 23626738
  39. BK-IS1 (44-113) alpha subunit has been over-expressed using a bacterial system and purified in the presence of detergent micelles for multidimensional heteronuclear nuclear magnetic resonance (NMR) structural studies. PMID: 23831469
  40. BK(Ca) as a crucial part of the signaling cascade of LH/hCG in Leydig cells. PMID: 23267835
  41. These results strongly suggest that cav3 possesses direct interaction with KCa1.1, presumably at the same domain for cav1 binding. PMID: 23237801
  42. whole-cell current, spontaneous transient outward current, and Ca(2+) sensitivity of BK(Ca) channels are reduced in human vascular smooth muscle cells. This may account for the dysfunction of artery relaxation in primary hypertension. PMID: 23232643
  43. enhanced expression of KCNMA1 correlates with and contributes to high proliferation rate and malignancy of breast cancer. PMID: 22899999
  44. Phosphodiesterase blockade causes relaxation of human urinary bladder smooth muscle by increasing transient K(Ca)1.1 channel current activity, hyperpolarizing cell membrane potential, and decreasing the global intracellular Ca(2+). PMID: 22896041
  45. TM1 of the beta2-subunit physically binds to the transmembrane S1 domain of the alpha-subunit of BK potassium channels PMID: 22710124
  46. The effects of protein kinase C on Slo surface expression might be indirect; when clustered on hair cell membranes, Slo contributes to increasing amounts of channel clusters on high-frequency responsiveness of hair-cell membranes. PMID: 22538239
  47. Multiple cholesterol recognition/interaction amino acid consensus (CRAC) motifs in cytosolic C tail of Slo1 subunit determine cholesterol sensitivity of Ca2+- and voltage-gated K+ (BK) channels. PMID: 22474334
  48. These studies identify for the first time a central role for beta-catenin in mediating Slo surface expression. PMID: 22194818
  49. BK(Ca) channels are involved in the response of podocytes to chronic hypoxia via the upregulation of the beta4-subunit. PMID: 22446331
  50. Cym04 reversibly left-shifts the Slo1 half-activation voltage. It interacts with the S6/RCK linker & mimics site-specific shortening of this passive spring, transmiting force from the cytosolic gating ring structure opening the channel's gate. PMID: 22331907

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Involvement in disease Paroxysmal nonkinesigenic dyskinesia, 3, with or without generalized epilepsy (PNKD3)
Subcellular Location Cell membrane; Multi-pass membrane protein.
Protein Families Potassium channel family, Calcium-activated (TC 1.A.1.3) subfamily, KCa1.1/KCNMA1 sub-subfamily
Tissue Specificity Widely expressed. Except in myocytes, it is almost ubiquitously expressed.
Database Links

HGNC: 6284

OMIM: 600150

KEGG: hsa:3778

UniGene: Hs.144795

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