Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

CALCOCO2

Uniprot No.

Q13137

Research Area

Immunology

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-446aa

Target Protein Sequence

MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

58.3 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion.

Gene References into Functions

Backfolding of MVI regulates its ability to bind DNA and that a putative transcription co-activator NDP52 relieves the auto-inhibition of MVI to enable DNA binding. Additionally, we show that the MVI-NDP52 complex binds RNAPII, which is critical for transcription, and that depletion of NDP52 or MVI reduces steady-state mRNA levels. PMID: 29187741
findings reveal a negative feedback loop of RLR signaling generated by Tetherin-MARCH8-MAVS-NDP52 axis and provide insights into a better understanding of the crosstalk between selective autophagy and optimal deactivation of type I IFN signaling. PMID: 28965816
The authors propose that Rab35-GTP is a critical regulator of autophagy through recruiting autophagy receptor NDP52. PMID: 28848034
The authors identify calcium-binding and coiled-coil domain 2 (CALCOCO2, also known as NDP52) as a binding partner of influenza A virus PB1-F2. PMID: 28613140
Presence of the NDP52 rs2303015 minor variant increases the risk for spontaneous bacterial peritonitis in patients with alcoholic cirrhosis. PMID: 26493630
insight into how CALCOCO2 targets ubiquitin-decorated pathogens for autophagic degradations PMID: 26506893
control retrotransposon insertion in the genome PMID: 25366815
These data show that NDP52 promotes the maturation of autophagosomes via its interaction with LC3A, LC3B, and/or GABARAPL2 through a distinct LC3-interacting region, and with MYOSIN VI. PMID: 25771791
data support a model in which non-ND10 resident Sp100 acts as a negative regulator of polycomb repressive complex-2 (PRC2) recruitment, and suggest that KSHV may actively escape ND10 silencing mechanisms to promote establishment of latent chromatin. PMID: 25033267
the role of ORF75 in the antagonization of ND10-mediated intrinsic immunity PMID: 24453968
Dimeric NDP52 forms a ternary complex with two monomeric galectin-8 molecules as well as two LC3C molecules PMID: 23511477
The missense SNP rs2303015 (Val248Ala) in the NDP52 was associated with Crohn's disease. PMID: 23624108
Data indicate that the binding site in galectin-8 is essential for the recruitment of the autophagy receptor NDP52 to cytosol-exposed Salmonella Typhimurium. PMID: 23386746
Autophagy of Tax1bp1/Ndp52 promotes non-canonical NF-kappaB signalling. PMID: 23209807
Data show that the selectivity of the autophagy receptor NDP52 for LC3C is crucial for innate immunity since cells lacking either protein cannot protect their PMID: 23022382
Data show that the miRNA-processing enzyme, DICER and the main miRNA effector, AGO2, are targeted for degradation as miRNA-free entities PMID: 23143396
a selective autophagic mechanism mediated by NDP52 that works downstream of TRIF-TRAF6. PMID: 21964925
p62 and NDP52 act cooperatively to drive efficient antibacterial autophagy by targeting the protein complexes they coordinate to distinct microdomains associated with bacteria PMID: 21079414
p62 and NDP52 proteins target intracytosolic Shigella and Listeria to different autophagy pathways. PMID: 21646350
ND10 and ND10 components might be important defensive factors against the CMV cross-species infection PMID: 21552525
interacts with ICP4 regulatory protein of HSV-1 during early stage of infection PMID: 14747555
NDP52 was identified as a myosin VI binding protein; results suggest that myosin VI-T6BP-NDP52 complexes may play a role in coordinating cytokine signalling and membrane transport pathways with actin filament organisation and cell adhesion PMID: 17635994
NDP52 recognizes ubiquitin-coated Salmonella enterica and recruits TBK1. PMID: 19820708

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Subcellular Location

Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Cytoplasmic vesicle, autophagosome membrane; Peripheral membrane protein.

Protein Families

CALCOCO family

Tissue Specificity

Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.

Database Links

HGNC: 29912

OMIM: 604587

KEGG: hsa:10241

STRING: 9606.ENSP00000258947

UniGene: Hs.514920

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Code	CSB-EP614394HU
MSDS	MSDS
Size	$306
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Calcium-binding and coiled-coil domain-containing protein 2 (CALCOCO2)

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