Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
CEACAM4; CGM7Carcinoembryonic antigen-related cell adhesion molecule 4; Carcinoembryonic antigen CGM7; Non-specific cross-reacting antigen W236
Species
Homo sapiens (Human)
Expression Region
36-155aa
Target Protein Sequence
FTIEALPSSAAEGKDVLLLACNISETIQAYYWHKGKTAEGSPLIAGYITDIQANIPGAAYSGRETVYPNGSLLFQNITLEDAGSYTLRTINASYDSDQATGQLHVHQNNVPGLPVGAVAG
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 36-155 form the expressed segment for recombinant Human CEACAM4. This CEACAM4 protein is expected to have a theoretical molecular weight of 16.7 kDa. The CEACAM4 protein was expressed in e.coli. The N-terminal 6xHis tag was fused into the coding gene segment of CEACAM4, making it easier to detect and purify the CEACAM4 recombinant protein in the later stages of expression and purification.
Carcinoembryonic antigen-related cell adhesion molecule 4 (CEACAM4) is mainly studied in the field of male reproductive health. CEACAM4 is a cell adhesion molecule found on the surface of sperm cells, and studies emphasize its significance in mediating interactions between sperm and other cells within the male reproductive system. Researchers are delving into the molecular mechanisms by which CEACAM4 contributes to sperm-egg binding and fertilization, aiming to unravel insights that could have implications for fertility treatments and male reproductive health. While the primary focus is on male reproductive biology, CEACAM4 might also have relevance in broader areas such as cell adhesion research and molecular biology.
