Recombinant Human Caspase recruitment domain-containing protein 11(CARD11) ,partial

Code CSB-YP866306HU
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Source Yeast
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Code CSB-EP866306HU
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Source E.coli
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Code CSB-EP866306HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866306HU
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Source Baculovirus
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Code CSB-MP866306HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names CARD11
Uniprot No. Q9BXL7
Alternative Names 0610008L17Rik; 2410011D02Rik; Bcl10 interacting maguk protein 3; BIMP 3; BIMP3; CAR11_HUMAN; CARD 11; CARD containing MAGUK protein 3; Card maguk protein 1; CARD-containing MAGUK protein 1; CARD11; CARD11 protein; Carma 1; CARMA1; Caspase recruitment domain containing protein 11; Caspase recruitment domain family member 11; Caspase recruitment domain-containing protein 11; MGC133069
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Adapter protein that plays a key role in adaptive immune response by transducing the activation of NF-kappa-B downstream of T-cell receptor (TCR) and B-cell receptor (BCR) engagement. Transduces signals downstream TCR or BCR activation via the formation of a multiprotein complex together with BCL10 and MALT1 that induces NF-kappa-B and MAP kinase p38 (MAPK11, MAPK12, MAPK13 and/or MAPK14) pathways. Upon activation in response to TCR or BCR triggering, CARD11 homooligomerizes to form a nucleating helical template that recruits BCL10 via CARD-CARD interaction, thereby promoting polymerization of BCL10 and subsequent recruitment of MALT1: this leads to I-kappa-B kinase (IKK) phosphorylation and degradation, and release of NF-kappa-B proteins for nuclear translocation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Promotes linear ubiquitination of BCL10 by promoting the targeting of BCL10 to RNF31/HOIP. Stimulates the phosphorylation of BCL10. Also activates the TORC1 signaling pathway.
Gene References into Functions
  1. CARMA1 forms CARMA1-BCL10-MALT1-TRAF6 signalosome.CARMA1, the BCL10 nucleator, serves as a hub for formation of star-shaped filamentous networks of BCL10 and significantly decreases the lag period of BCL10 polymerization. PMID: 29382759
  2. study results suggest variations in the inflammasome, particularly in NLRP1 and CARD11, may be associated with chronic Chagas cardiomyopathy PMID: 29438387
  3. CARD11, a scaffold protein required for B-cell receptor (BCR)-induced NF-kappaB activation, was screened in an additional 173 MCL samples and mutations were observed in 5.5% of cases. PMID: 27224912
  4. CARMA1 may be aberrantly upregulated in T-ALL, leading to disease progression and migration of leukemic cells to the CNS PMID: 27698445
  5. findings indicate that a single hypomorphic mutation in CARD11 can cause potentially correctable cellular defects that lead to atopic dermatitis PMID: 28628108
  6. NF-kappaB and beta-catenin signaling by gain-of-function mutations in CARMA1 augments WNT stimulation and is required for regulating the expression of distinct NF-kappaB target genes to trigger cell-intrinsic and extrinsic processes that promote DLBCL lymphomagenesis PMID: 26776161
  7. CARD11 mutations are rare in patients with CD5+ DLBCL. PMID: 27915469
  8. results define molecular determinants that control the production of Lin(Ub)n-Bcl10, an important signaling intermediate in TCR and oncogenic CARD11 signaling. PMID: 27777308
  9. We evaluated the relationship between rs4722404 polymorphism in the CARD11 gene, and the risk and clinical features of psoriasis vulgaris in a southern Chinese Han cohort.We identified no association between the SNP and risk of psoriasis vulgaris. PMID: 27421022
  10. single amino acid oncogenic CARD11 mutations can perturb or bypass the action of redundant inhibitory REs to achieve the level of hyperactive CARD11 signaling required to support lymphoma growth. PMID: 26884334
  11. Cooperative Control of Caspase Recruitment Domain-containing Protein 11 (CARD11) Signaling by an Unusual Array of Redundant Repressive Elements. PMID: 26884335
  12. CARMA1- and MyD88-dependent activation of Jun/ATF-type AP-1 complexes is a hallmark of ABC diffuse large B-cell lymphomas. PMID: 26747248
  13. Data show that caspase recruitment domain-containing protein 11/B-cell CLL/lymphoma 10/mucosa-associated lymphoid tissue lymphoma translocation gene 1 signaling drives lymphoproliferation through NF-kappa B and c-Jun N-terminal kinase activation. PMID: 26668357
  14. CARD11-mediated alterations in NF-kappaB signaling may be an early event in the development of cutaneous squamous cell carcinoma PMID: 26212909
  15. Taken together, our data indicate that miR-539 is a novel regulator of migration and invasion in human thyroid cancer cells by targeting CARMA1. PMID: 26206083
  16. CARMA1 clustering through SH3-GUK domain interactions is required for its activation of NF-kappaB signalling PMID: 25602919
  17. Both carried homozygous germline mutations in CARD11 (p.Cys150*), impairing NF-kappaB signaling and IL-2 production PMID: 26289640
  18. Three patients have been described with mild B-cell lymphocytosis and a CARD11 C49Y missense mutation. PMID: 25930198
  19. Overexpression of CARMA-BCL10-MALT in T-ALL may contribute to the constitutive cleavage and inactivation of A20, which enhances NF-kappaB signaling and may be related to T-ALL pathogenesis. PMID: 25384343
  20. Data indicate that caspase recruitment domain family, member 11 protein CARD11 has complex 5'UTRs and is sensitive to eIF4A RNA helicase inhibition. PMID: 25320244
  21. Data indicate that caspase recruitment domain membrane-associated guanylate kinase protein 1 (CARMA1)-silenced cells K562/shCARMA 1-93 showed the greatest inhibition of CARMA 1 gene and protein expressions. PMID: 25001930
  22. CKIP-1 interacts with CARMA1 and has an inhibitory effect on PKCtheta;-CBM-NF-kappaB signaling. PMID: 24465689
  23. Data indicate that phosphorylation of Carma1 down-stream of TCR/CD28-dependent NF-kappaB induction is regulated in part by Akt. PMID: 24548923
  24. CARMA1 CARD was then purified to homogeneity and crystallized at 293 K. Finally, X-ray diffraction data were collected to a resolution of 3.2 A from a crystal belonging to space group P2(1)2(1)2(1) PMID: 23545653
  25. Combining crystallography, nuclear magnetic resonance, and electron microscopy, we reveal the structure of the Bcl10 CARD filament and the mode of interaction between CARMA1 and Bcl10 PMID: 24074955
  26. SMO activates trimeric G proteins and CARMA1-associated signaling complex, leading to NF-kappaB activation and results in diffuse large B-cell lymphoma. PMID: 23632891
  27. distinct CARMA1-dependent control of key cell cycle proteins in T cells is coordinated by ADAP PMID: 22411628
  28. In patients with CARD11 deficiency, the combination of impaired activation and especially upregulation of inducible T-cell costimulator on T cells. PMID: 23374270
  29. These data provide the first evidence that ubiquitination of CARMA1 by STUB1 promotes TCR-induced NF-kappaB signaling. PMID: 23322406
  30. CARD11 gain-of-function mutations selectively confer the ability to associate with Bcl10 and induce K63-linked ubiquitination of Bcl10. PMID: 23149938
  31. The incidence of CARD11 mutations was 10.7% in Middle Eastern diffuse large B-cell lymphoma. PMID: 22397314
  32. Findings show that regulation of CARD11 signaling is a critical switch governing the decision between death and proliferation in antigen-stimulated mature B cells. PMID: 23027925
  33. CARD11 mutations may predispose to B but not T lymphoid malignancy. PMID: 23129749
  34. The present study failed to find any mut-ation in MYD88, CARD11 or CD79B in ocular MALT lymphoma. PMID: 22808296
  35. PKCdelta is a negative regulator in T cell activation through inhibiting the assembly of CARMA1 signalosome. PMID: 22528498
  36. TLR-dependent TRAF6-MKK3-p38 MAPK signaling pathway synergizes with PKCtheta;-MEK-ERK signaling pathway. CARMA1 plays a crucial role in mediating this synergistic effect via TRAF6. PMID: 22303480
  37. In Jurkat cells, CARMA1 is required for induction of Th2 transcription factors, GATA3 and JunB, and controls IL-4, IL-5, and IL-10 production. PMID: 22371397
  38. expression of CARMA1 mRNA is likely associated with the expression of MUM1 and shows male predominance in diffuse large B cell lymphoma. PMID: 21569705
  39. A combination of in vitro and in vivo studies demonstrates that the CARMA1 transgene is required for optimal T cell responses to T cell receptor engagement and development of allergic airway inflammation, after activation of T cells in a murine model. PMID: 22075698
  40. genetic polymorphism is associated with common variable immunodeficiency PMID: 21905497
  41. Very low mutation frequency of exons 5-9 in the CARD11 gene from 186 adult acute leukemia and 31 multiple myeloma samples. PMID: 21176849
  42. PP2A-mediated dephosphorylation of Carma1 is a critical step to limit T-cell activation and effector cytokine production. PMID: 21157432
  43. CARM1 is transcriptional coactivators that deposit H3R17me2a and H4R3me2a marks, respectively. PMID: 21172665
  44. results establish a mechanism that explains how diffuse large B cell lymphoma-associated mutations in CARD11 can initiate spontaneous, receptor-independent activation of NF-kappaB PMID: 20799731
  45. The mutations of the oncogene CARD11 may contribute to NF-kappaB activation and thereby play a role in the pathogenesis of Primary CNS lymphoma. PMID: 20544211
  46. the ADAP CARMA1 binding site is required for IKK gamma ubiquitination; both TAK1 and CARMA1 binding sites are required for IkappaB alpha phosphorylation and degradation and NF-kappaB nuclear translocation PMID: 20164171
  47. CARMA1 is an essential signaling component that mediates TCR-induced NF-kappa B activation. PMID: 12154356
  48. CARMA1 is a critical lipid raft-associated regulator of TCR-induced NF-kappa B activation and CD28 costimulation-dependent Jnk activation. PMID: 12154360
  49. CARMA1 and CARMA3 bind to Ikappa kinase gamma-NFkappaB in B and T lymphocytes PMID: 15184390
  50. Phosphorylation of CARMA1 plays a critical role in T Cell receptor-mediated NF-kappaB activation. PMID: 16356856

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Involvement in disease B-cell expansion with NFKB and T-cell anergy (BENTA); Immunodeficiency 11 A (IMD11A); Immunodeficiency 11B with atopic dermatitis (IMD11B)
Subcellular Location Cytoplasm. Membrane raft.
Tissue Specificity Detected in adult peripheral blood leukocytes, thymus, spleen and liver. Also found in promyelocytic leukemia HL-60 cells, chronic myelogenous leukemia K-562 cells, Burkitt's lymphoma Raji cells and colorectal adenocarcinoma SW480 cells. Not detected in H
Database Links

HGNC: 16393

OMIM: 607210

KEGG: hsa:84433

STRING: 9606.ENSP00000380150

UniGene: Hs.648101

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